1) retroviral transduction system
反转录病毒转导系统
2) retrovirus
[英][,retrəu'vaiərəs] [美][,rɛtrə'vaɪrəs]
反转录病毒
1.
To explore the cell lineage tracing methods in pancreas in vivo and the regenerate cells source,we used pseudo-type retrovirus to transfect adult mouse pancreas which had been partially pancreatectomized by rubbing the kerf using a cotton stick saturated with retrovirus suspension then injecting 100μL retrovirus suspension into pancreas,injecting 100μL retrovirus by caudal vein,or .
为了探讨胰腺活体细胞世系追踪的方法和胰腺损伤后再生细胞的来源,分别通过胰腺伤口涂抹并胰内注射、尾静脉注射及腹腔注射三种方法,利用假型反转录病毒对成体小鼠大部分切除后胰腺的细胞进行世系追踪。
2.
The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth.
运用反转录病毒介导的插入突变技术 ,在脊椎动物斑马鱼中已经获得了许多影响胚胎发育和细胞生长过程的突变体 ,并找到了对应的基因。
3.
Methods ①ADAR1 gene was amplified using PCR from mouse cDNA and ligated into retrovirus vector, MIEV.
方法 ①构建反转录病毒载体 :PCR从小鼠cDNA中放大ADAR1全长基因 ,连入反转录病毒MIEV载体 ,感染包装细胞后分泌病毒颗粒 ;②病毒颗粒感染T淋巴细胞CTLL2和B淋巴细胞A2 0 ,观察细胞增殖的变化 ;③MTT法测定细胞增殖率。
4) retrovirus vector
反转录病毒载体
1.
Construction of retrovirus expressing β-scretase substrate-base peptide and its steady packaging cell line mediated by pLXSN retrovirus vector;
β-分泌酶底物肽反转录病毒载体及其包装细胞株的构建
2.
Cloning of bovine sox2 gene and construction of its retrovirus vector
牛sox2基因的克隆及重组反转录病毒载体的构建
3.
This review focused on Haemophilia B s mechanism and the new development of retrovirus vector used in the gene therapy of Haemophilis B.
本文介绍了血友病B的发病机理以及以反转录病毒载体为工具进行血友病B基因治疗的研究进展。
6) bovine retrovirus
牛反转录病毒
1.
Here, Three kinds of bovine retrovirus including BIV, BFV and BLV were selected for characterization of BICP0 activation.
为探究其激活特性,选取三种典型牛反转录病毒BIV、BLV和BFV,对其LTR上NF-κB及AP-1位点进行单独或共缺失,通过Luc瞬时表达分析发现:BICP0可激活所选三种牛反转录病毒的启动子,且激活功能与其NF-κB和AP-1位点有关。
补充资料:反转录转座子
分子式:
CAS号:
性质:又称反转录转座子。指一类具有反转录功能的可移动遗传因子。如醇母中的Tyl因子,它所含的遗传信息可从DNA→RNA→DNA以反转录方式流动。
CAS号:
性质:又称反转录转座子。指一类具有反转录功能的可移动遗传因子。如醇母中的Tyl因子,它所含的遗传信息可从DNA→RNA→DNA以反转录方式流动。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条