1) UL 674
L6 74
2) Yan 74(China,Changyu)
烟74
4) L6 myoblast
L6细胞
1.
After retrieving the PCR products,ligating it with pTARGET~(TM) vector,transformating ligation reaction to JM109 huge efficiency competent cells and identifying the recombinant plasmid,the recombinant eukaryotic expression vector pTARGET~(TM)-AANAT was transfected into rat L6 myoblast.
RT-PCR及Western blotting检测表明,转化后的L6细胞表达AANAT mRNA,细胞具备AANAT蛋白的表达能力。
2.
Objective:To construct a eukaryotic expression vector system of rat NNNAT gene,transfect it into L6 myoblast,and entrap engineered cells into APA microcapsule for preparing artificial pineal tissue.
方法:RT-PCR技术扩增大鼠AANATcDNA,酶切连接pTARGETTM载体,脂质体法转染L6细胞并检测pTARGETTM-AANAT的基因表达产物;将高表达活性的L6细胞进行APA微囊化处理,倒置显微镜观察微囊形态,Western-blot检测囊内细胞AANAT蛋白表达活性。
5) L6 myoblast cells
L6细胞株
6) Zhuang74 block
桩74块
补充资料：1H-Pyrazole-3-carboxylic acid, 4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-, sodium salt C.I. Reactive orange 74 5-dihydro-5-oxo-1-(4-sulfophenyl)-]phenyl]azo]- sodium salt 1H-Pyrazole-3-carboxylic acid,4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-,sodium salt
CAS：85536-88-5
分子式：C20H20N4O14S3·xNa
中文名称：C.I.活性橙74
英文名称：1H-Pyrazole-3-carboxylic acid, 4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-, sodium salt C.I. Reactive orange 74 5-dihydro-5-oxo-1-(4-sulfophenyl)-]phenyl]azo]- sodium salt 1H-Pyrazole-3-carboxylic acid,4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-,sodium salt
分子式：C20H20N4O14S3·xNa
中文名称：C.I.活性橙74
英文名称：1H-Pyrazole-3-carboxylic acid, 4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-, sodium salt C.I. Reactive orange 74 5-dihydro-5-oxo-1-(4-sulfophenyl)-]phenyl]azo]- sodium salt 1H-Pyrazole-3-carboxylic acid,4-[[2,5-dimethoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-,sodium salt
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条