Quantitative study on distribution and latency sites of duck plague virus;

The symptom and pathological change of the disease is as same as duck plague,but mainly occurred in duckling,while adult duck less infected.

The propagation characteristics of virulent duck plague virus(DPV)in duck embryo fibroblast(DEF)were studied by the method of light microscopy observation of DEF cell culture monolayer,electron microscopy observation of infected DEF cell culture,real-time PCR detecting virus propagation.

Dynamics of IFN-α mRNA expression in liver of ducks infected with duck plague virus of different virulence;

Purification and electron microscopy observation after negative staining of duck plague virus;

Prokaryotic expression of N-terminal antigenic domain of duck plague virus gB protein and the establishment of putative indirect ELISA assay;

The SD-01 strain of DPV was propagated in chicken embryo fibroblast monolayer cells.

According to the UL6 and UL7 gene data of duck plague virus(DPV),a pair of primers were designed and used for a polymerase chain reaction(PCR) with two duck plague vaccine strains and one standard strain and one field isolate.

According to DPV gC gene sequence discovered by our laboratory,a series of scientific research were conducted and the results were obtained as followed: 1.

Detetion of antibodies against new duck plague virus by an indirect ELISA;