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1)  GC clamp
GC发卡结构
2)  Double hairpin motif
双发卡结构
3)  short hairpin RNA
短发卡结构状RNA
4)  small hairpin RNA
小发卡结构RNA
1.
Methods Genome sequences of Survivin gene was retrieved from gene bank,siRNA(small interfering RNA) was designed according to the Tuschl s principle of RNAi-based medicine,and was converted into cDNA coding expression of shRNA(small hairpin RNA) of siRNA for Survivin gene.
方法根据GeneBank数据库提供的Survivin基因所有变异体的共同核苷酸序列,按照Tuschl设计原则,选择设计双链siRNA,再转化为能表达其小发卡结构RNA(small hairpinRNA,shRNA)的DNA序列,并与载体pSINsi-hU6定向连接,构建受控于启动子U6的真核表达载体pSIN/shRNA1、pSIN/shR-NA2,经限制性内切酶酶切和DNA测序进行鉴定。
5)  small hairpin interfering RNA
发卡结构小片段RNA
1.
Methods: Eukaryotic vector mU6pro was used to construct herg gene-targeting small hairpin interfering RNA plasmid: shRNA-herg.
目的:探讨发卡结构小片段RNA(small hairpin interfering RNA,shRNA)干扰人ERG(human ether-α-go-go-related gene,HERG)钾通道治疗裸鼠皮下人成神经细胞移植瘤的疗效和作用机制。
6)  short hairpin ribonucleic acid
短发卡结构状核糖核酸
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