L-1 could increase tip meristem culture plant let ratio to 11% ~ 17% and reduce average plantlet time by 7~10 days.

Study on Stem Apex Tissue Culture of Zingiber officinale Rosc. and the Control of Contamination Rate;

The system of high-effective regeneration and propagation of apical meristem from Lycium Barbarum was established by using apical meristem as explants.

T gene to inoculate lower plumular axes and apical meristem of four cotton cultivars, which are Lumian No.

Sufficient plantlets of regeneration were produced within 3～6 weeks induced and culture from apical meristems.

The apical meristems were excised from the seedlings of 40～60 hours and cultured on the MSB medium without/with hormone or active charcoal.

On the modified MS medium supplemented with suitable concentrations of 6-BA(N6-benzyladenine),shoot tip meristem from different genotypes of maize were inoculated for inducing callus culture and then for bud development culture,each culture stage lasted for four weeks,multiple shoot clumps and regenerated plantlets could be highly frequency produced,3～8 buds could derived from one original tip.

Genetic transformation of cotton shoot apical meristem via gene-gun bombardment is advantageous over Agrobacterium-meditated transformation and pollen-tube pathway in the genotype-independent and time-consuming.