1) method of tissue block
酶消化培养法
1.
In this experi-ment the method of enzymatic digestion and the method of tissue block were combined to prepare the feeder layer of MEFthat on a certain degree Solved this problem.
本实验通过将组织块培养法和酶消化培养法结合起来制备小鼠胎儿成纤维细胞饲养层,在一定程度上解决了这一问题。
2) method of trypsinization
胰酶消化植块培养法
1.
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
3) enzyme digestion
酶消化法
1.
METHODS:Freshly extracted human permanent teeth were collected and sectioned under cooling ECS solution and then the odontoblasts were isolated through enzyme digestion.
方法:选取新鲜拔除的健康恒牙,利用牙科专用慢速切锯制备牙齿切片,采用酶消化法分离出完整的人成牙本质细胞,利用RT-PCR技术直接从人成牙本质细胞中克隆L型钙离子通道α1亚基D亚型特异性基因片段,将其亚克隆入pGM-T载体进行序列测定。
2.
Methods The sertoli cells in rats testicular were obtained through multiple enzyme digestion and these cells were cultivated in vitro to maintain their favorable activities.
方法利用多重酶消化法,获取睾丸Sertoli细胞,进行体外原代培养以维持其良好的活性。
3.
Results:The HOBs purified by enzyme digestion in vitro had a normal function showing synthesized alkaline phosphatase(ALP)and forming calcified nodules as observed in vivo,and significant difference in optical density(OD490) was found between morphine group with different concentrations and the control group(P<0.
方法:用胰酶-胶原酶消化法从正常女性松质骨中分离培养成骨细胞,用茜素红染色法、改良Gomonri钙钴法鉴定成骨细胞;制备不同浓度的吗啡及其拮抗剂纳洛酮,作用于体外培养的成人正常成骨细胞上,通过四甲基偶氮唑盐(MTT)比色法检测吗啡对其的影响;通过RT-PCR法半定量分析不同浓度的吗啡及其拮抗剂纳洛酮对成骨细胞骨钙素(BGP mRNA)表达的影响。
4) dissociated culture
消化分离培养
1.
Spiral ganglion neurons were cultured by dissociated culture method or explants culture method.
方法:出生4-6天的Sprague-Dawley大鼠,解剖出蜗轴,分别进行组织块培养和消化分离培养,铺片方法采取单纯层粘连蛋白(Laminin)、单纯多聚赖氨酸(Poly-D-lysine,PDL)、多聚赖氨酸加层粘连蛋白(PDL+Laminin)包被、单纯鼠尾胶原蛋白包被和不包被等5种方法,观察螺旋神经的神经突的生长情况。
5) One enzyme digestion method
单酶消化法
6) Cold enzyme digestion
冷酶消化法
补充资料：胨[番木瓜蛋白酶消化大豆粉制备]
CAS: 91079-46-8
中文名称: 胨[番木瓜蛋白酶消化大豆粉制备]
英文名称: Peptone from soya bean flour papain-digested
中文名称: 胨[番木瓜蛋白酶消化大豆粉制备]
英文名称: Peptone from soya bean flour papain-digested
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条