Selective retention plays a critical role in the realization of animal cells perfusion culture with highviability and concentration.

The space environment refers to an environment far away from the earth s surface and different in many respects from the surface of the earth,where animal cells will be influenced by all kinds of space factors,including mainly space radiation,extreme temperature,high vacuum,micro-magnetic field and the special environment in spacecrafts,namely,that of microgravity.

We reviewed the efforts made in this direction,and how the metabolic engineering of animal cells has been approached to reshape their profiles in various key aspects,namely:central metabolism,protection of apoptosis,regulation of cell cycle,and finally,the combined engineering of different aspects.

Research advance in large-scale culture of animal cells;

Many biological products were manufactured by means of large-up culture of animal cells.

The growth of animal cells in vitro needs many kinds of growth factors.

The cellular mechanism of henogenesis for cellulous plant;

The Doushantuo Period of the Ediacaran (Sinian) is not only an important epoch of the origin and development of the mulicellular organisms and the accumulation of phosphates, but also an important epoch of the Rodinia Supercontinent cracking and a tempestuous epoch of the chemical, climatic, and environmental changes.