1) gene expression cassette
基因表达盒
1.
A gene expression cassette with molecular size of the insert, a recombinant plasmid containing the PK gene of PRV,equal to vector was cloned through partial repair of the terminal ends of insert and vector, thus transforming them from nonadherent to adherent ones.
以含有伪狂犬病毒 (pseudorabiesvirus ,PRV)蛋白激酶 (proteinkinase ,PK)基因的重组质粒为基础 ,利用非互补粘性末端经过部分补平后成为粘性末端 ,再进行连接的方法克隆了与载体分子大小相当的报告基因表达盒 用限制性内切酶分析确证了伪狂犬病毒表达载体中报道基因表达盒的插入方向 ,并通过导入细胞后检测外源基因在体外的瞬时表达 ,直接鉴定了表达载体的生物学活
2) bar gene cassette
bar基因表达盒
1.
In the present article, studies related to the hereditary behavior of bar gene cassette in T1 to T3 generations of the transgenic rice (Oryza sativa L.
本文研究了基因枪转化的bar基因表达盒在转基因水稻T1～T3世代中的遗传行为。
2.
The recombination structure of bar gene cassette(containing the promoter,coding region and terminator) in rice(Oryza sativa L.
应用Southern杂交和引物组合PCR技术研究了基因枪转化的bar基因表达盒(包括启动子、编码区和终止子)在水稻(Oryza sativa L。
3) gene expression
基因表达
1.
Alterations of gene expression profiles in lungs of rats following sulfur dioxide inhalation;
二氧化硫吸入致大鼠肺组织基因表达谱改变
2.
Effect of heroin on gene expression of nucleotides catabolism in C6 glioma cell;
海洛因对C6细胞嘌呤核苷酸分解代谢基因表达的影响
3.
Effect of LYS polysaccharides on dementia-related gene expressions in SAMP8 mouse brain;
龙眼参多糖对SAMP8鼠脑组织痴呆相关基因表达的影响
4) expression
[英][ɪk'spreʃn] [美][ɪk'sprɛʃən]
基因表达
1.
Effects of environmental estrogen diethylstilbestrol on expression of WISP-2 gene in human breast cell;
已烯雌酚与人乳腺癌细胞WISP-2基因表达
2.
Study on Expression of Recombinant Plasmid PcDNA3.1-AMG for Human Amelogenin in Muscle Tissues of Mice;
釉原蛋白基因表达质粒PcDNA3.1-AMG在小鼠肌肉组织中表达的研究
3.
Objective: To design and synthesize codon-optimized MoPn-maior outer membrane protein (MOMP) gene(HuMOMP),construct recombinant eukaryotic expression plasmids of HuMOMP gene and EGFP-HuMOMP fusion gene.
目的:设计合成优化密码沙眼衣原体主要外膜蛋白(MOMP)基因,克隆构建优化密码MOMP(HuMOMP)基因的真核表达质粒及EGFP-HuMOMP融合基因表达质粒。
5) expression of gene
基因表达
1.
The effects of high concentration Adenosine on the expression of gene coded by mitchondia after reperfusion;
高浓度腺苷对脑缺血再灌注线粒体编码基因表达的影响
2.
The effects of high concentration outside ATP on the expression of gene coded by mitchondia after reperfusion;
高浓度外源ATP对脑缺血再灌注线粒体编码基因表达的影响
3.
Study on expression of genes in lung cancer cells A549 treated by inducer of differentiation;
结论全反式维甲酸、亚硒酸钠和三氧化二砷能引起A549肿瘤相关基因表达的改变,诱导分化药物对肺癌的作用机制表现为增殖抑制、促进凋亡、周期阻滞、转移抑制、诱导分化敏感度增加等多方面的改变。
6) Genetic Expression
基因表达
1.
In order to explore influence of static training on β endorphin and POMC genetic expression in rats, 168 rats were randomly divided into six groups: high load (A), medium load (B), low load (C) group, dynamic training (D) group, banding (E) group and normal (F) group.
探索静力训练对大鼠 β 内啡肽及其前体前阿黑皮素 (POMC)基因表达的影响。
2.
The differential display reverse transcription-PCR and suppressed-subtractive-hybridi have been extensively used in the difference analysis of genetic expression because of their high sensitivity and lower fasle positive rate.
差异显示反转录 -PCR和抑制性扣除杂交是目前广泛应用于基因表达差异分析的方法 ,它们分别具有灵敏度高假阳性率低等特点 ,对其原理、技术、优缺点及在基因表达差异分析中的应用进行简要概
3.
Objective: To investigate the mechanism of artherosclerosis and plaque disruptioninduced by disfunction of vascular endothelial cell with hyperhomocysteinaemia , we observe the effects of homocysteine on the genetic expression of matrix metalloproteinases(MMPs) in human vascular endothelial cell in 1, 6, 24 hours.
目的:观察不同浓度的同型半胱氨酸(homocysteine,Hcy)在1、6、24小时对血管内皮细胞(vein endothelia cell,VEC)的基质金属蛋白酶(matrix metallopoeinases,MMPs)基因表达的影响,在基因表达水平探讨同型半胱氨酸损伤血管内皮细胞功能,诱发动脉粥样硬化和斑块破裂的相关机制。
补充资料：基因表达系列分析
基因表达系列分析（sage）是通过快速和详细分析成千上万个est（express sequenced tags）来寻找出表达丰富度不同的sage标签序列。再次访法中，通过限制性酶切可以产生非常短的cdna（10-14bp）标签，并通过pcr扩增和连接，随后对连接题进行测序。sage大大简化和加快了3’端表达序列标签的收集和测序。同dd一样，sage是一个“开放”的系统，可以发现新的未知的序列。在进行标本的比较之前，sage在cdna的产生和处理上需要较多个步骤。由于sage是一个依赖dna测序的基因计量方法，它对基因表达的测定比dd更加量化。由于需要进行大量的测序反应，所以费用因素使大多数研究机构对其广泛应用的主要限制。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条