One 134 bp fragment was amplified in anthers of male sterile and fertile wheat using one pair of degenerated primer designed based on the conserved domain of MS2 gene in Arabidopsis thaliana and Oryza sativa ,and one 1 604 bp male sterility gene homology sequence was extended by in silico cloning based on the 134 bp fragment.

Cloning and sequencing of disease resistance gene analogues in peach (Prunus persica L. Batch);

The primers weresubsequentlyused as PCR primers to amplify resistance gene analogues in a high blast resistant rice cultivar Yunxi 2 (Oryza sativa L.

Cloning and Analysis of Resistance Gene Analogs from Grape and Peach;

To analyze the presence of homologous sequences of ras in maize and rice, Southern blot hybridization was performed and positive signals was detected in these two species genomes.

To analyze the presence of homologous sequences of human tumor supprossor gene bcl 2 in maize and rice, Southern hybridization was performed and positive signals were detected in these two species genomes.