Glial fibrillary acidic protein ( GFAP) gene which is a novel porcine gene cloned and characterized by our laboratory recently was assigned regionally using pig × rodent somatic cell hybrid panel.

Objective To find out the suitable age of oocyte and parameters of electrical activation in the process of mouse somatic cloning.

Study on activation conditions of somatic cell cloning in Yanbian Yellow Cattle;

Research and applications of mammalian somatic cell cloning;

Preliminary study of cell fusion by PEG on handmade somatic cell cloning in cattle;

With the birth of Dolly sheep,the technology of somatic cell nuclear transfer is developing fast and many cloned animals are achieved recently.

In order to optimize the culture solution and improve the efficiency of somatic cell nuclear transfer,oxidative damage and developmental mode in vitro of reconstructed embryos from somatic cell nuclear transfer in Yanbian cattle were observed in the experiment treated with addition of H2O2 to the base culture solution(CR1aa + 5%FBS + 0.

In order to conserve our native excellent yellow cattle breeds,the somatic cell clone technique was studied,in which the bovine ear skin fibroblast in vitro cultured was used as nuclear donor.

Somatic cell clone has been applied successfully in sheep,goat,bovine,swine and so on.

Cloning by somatic nuclear transfer is an inefficient process in which many of the cloned animals die shortly after birth and display organ abnormalities.

The lung of somatic nuclear transfer cattle was observed by optical microscopy and HE staining techniques in this paper.