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1)  comparative genomic in situ hybridization (cGISH)
比较基因组原位杂交
1.
Molecular banding undertaken with comparative genomic in situ hybridization (cGISH) is a simple approach which generates chromosome characteristic signals in different species at regions with conserved repeated sequences.
利用玉米的近缘亲属摩擦禾的基因组DNA ,对玉米自交系 330、二倍体多年生类玉米及其远缘杂交孤雌生殖后代异源种质纯系 5 4 0的染色体进行比较基因组原位杂交 ,发现上述 3个供试种染色体上的异染色质纽因杂交信号极强而能被清楚地显示 。
2)  comparative genomic hybridization
比较基因组杂交
1.
Identification of the origin of marker chromosome by comparative genomic hybridization;
应用比较基因组杂交技术鉴定标记染色体的来源
2.
Analysis of chromosome variation of lymphocytes and comparative genomic hybridization of lung carcinoma in patients with long survival after operation;
高生存期肺癌患者外周淋巴细胞染色体及肺癌组织比较基因组杂交回顾性分析
3.
Comparative genomic hybridization study of two paclitaxel resistant ovarian carcinoma cell lines OC_3/PIX_3 and OC_3/PIX_5.;
卵巢癌紫杉醇耐药细胞株OC_3/PIX_3及OC_3/PIX_5的比较基因组杂交研究
3)  CGH
比较基因组杂交
1.
Results CGH results showed that the most interesting finding was the amplification of 2p22 in OC3/Tax300.
方法运用比较基因组杂交(comparativegenomichybridization,CGH)及RTPCR技术,分析卵巢癌紫杉醇耐药株染色体基因组变化和hmsh2基因在卵巢癌组织中的表达。
2.
Prostate cancer is a common disease among men but the knowledge of the prostate carcinogenesis is still limited,cDNA microarray-based comparative genomic hybridization(CGH) and expression profiling were performed to screen the genomic and the expression changes in prostate cancer respectively.
首先,cDNA阵列比较基因组杂交在>20%前列腺癌样本中检测到2q、3p/q、5q、6q、8q、9p、10p/q、11q、12p、14q、19p/q的扩增和1p、2p、4q、6p/q、7p、11p/q、12q、17p/q、19p/q、Xp/q的缺失。
3.
Methods:The surface-enhanced laser description/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and comparative genomic hybridization (CGH) were applied to the 4 patients with concurrent esophageal and gastric cardia cancers (CC),107 patsents with single esophageal cancer(EC) and 86 patients with gastric cardia cancer.
方法:采用表面增强激光解析离子化-飞行时间-质谱(SELDI-TOF-MS)与比较基因组杂交(CGH)技术分析比较食管贲门双源癌(4例)和单发食管癌(107例)、单发贲门癌(86例)患者血清蛋白质组和癌组织全基因组。
4)  genome in situ hybridization
基因组原位杂交
1.
By using genome in situ hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum × G.
该文以比克氏棉gDNA为探针,亚比棉异源四倍体根尖体细胞染色体为靶细胞染色体,封阻材料为亚洲棉(迁西小黑籽),进行亚比棉基因组原位杂交(Genomeinsituhybridization,GISH)及核型分析。
5)  genomic in situ hybridization
基因组原位杂交
1.
By genomic in situ hybridization (GISH) analysis,three T.
通过基因组原位杂交 (GISH)分析 ,在 5 9个小滨麦代换系M872 4 8 13与离果山羊草 (AegilopstriuncialisL) 3C染色体附加系的杂交后代中获得了 3株小麦 滨麦易位系 ,易位频率达到 5 0 8%。
2.
Genomic in situ hybridization (GISH) technique is a most effective method of detecting alien chromatin at molecular level.
基因组原位杂交是在分子水平上检测外源染色质的一种有效方法 ,其探针是总基因组DNA 。
3.
Genomic in situ hybridization (GISH) technique was established in Citrus to identify intergeneric sexual hybrid.
通过改进实验方法,建立起柑橘基因组原位杂交(Genomicinsituhybridization,GISH)分析技术,并成功地应用于属间有性杂种鉴定,为进一步分析柑橘体细胞杂种核基因组组成奠定了基础。
6)  genomic in situ hybridization (GISH)
基因组原位杂交
1.
Genomic in situ hybridization (GISH) analysis of B. oleracea and 5 related Brassica species;
甘蓝与芸薹属5个近缘物种的基因组原位杂交分析
2.
Genomic in situ hybridization (GISH) discriminates the A, B and C genomes in Brassica allotetraploid species;
基因组原位杂交辨别芸薹属异源四倍体AA、BB、CC基因组研究
3.
In this study,genomic in situ hybridization (GISH) was used to identify the chromosomal constitutions of the blue grained wheat Blue 58 originated from distant h.
应用基因组原位杂交技术 (GISH)对普通小麦 (TriticumaestivumL 。
补充资料:荧光原位杂交


荧光原位杂交


是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针,依据碱基配对原理,通过免疫细胞化学检测体系,在组织切片、细胞间期核或染色体等标本上,进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高,特异性强。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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