1) Rous sarcoma virus
禽劳斯肉瘤病毒
1.
The gp27 gene of 720 bp was amplified by RT-PCR method from SPF chicken and Chicken Embyo Fibroblast (CEF) which were infected with Rous sarcoma virus (RSV), and the gp27 gene was cloned into a plasmid veetor pUC19, the recombined plasmid were analyzed by restriction enzyme digestion and sequence analysis, the result showed that the cloning gene was the full-length target gene which has a 97.
以RT PCR方法从人工感染禽劳斯肉瘤病毒 (Roussarcomavirus ,RSV )SR RSV的SPF雏鸡体内及SPF鸡胚成纤维细胞 (CEF)培养物中分别扩增出 72 0bp的 gp2 7片段 ,将RT PCR产物重组到质粒载体 pUC19中 ,并对重组质粒进行酶切分析及序列测定。
2.
The gag p27 gene of 720bp was amplified by RT-PCR method from SPF chicken and Chicken Embryo Fibroblast(CEF) which were infected with Rous Sarcoma Virus (RSV), and the p27 gene was cloned into a plasmid vector pUC19 , the recombined plasmid were analyzed by restriction enzyme digestion and sequence analysis , the result shows that the cloning gene was the full-length larget gene which has a 97.
本试验以RT-PCR方法从人工感染禽劳斯肉瘤病毒(Rous Sarcoma Virus-,RSV)SR-RSV的SPF雏鸡体内及SPF鸡胚成纤维细胞(CEF)培养物中分别扩增出720bp的gag p27片段。
2) RSV
劳氏肉瘤病毒
3) Kirsten sarcoma virus (KiSV)
柯斯顿肉瘤病毒
6) Kristen murine sarcoma virus (KIMSV,KI-MSV)
柯斯顿鼠肉瘤(病毒)
补充资料：劳氏肉瘤病毒
分子式：
CAS号：
性质：又称Rous肉瘤病毒。美国病理学家劳斯(F. P. Rous)在1910年发现把禽类的恶性肉瘤的组织液经伯克菲尔德(素烧)滤筒(Berkefeld filter，一种硅藻土滤器)滤出液接种于另外一些正常鸡，结果发现这些正常鸡也生长出具有与病禽肉瘤一样的恶性肿瘤，并从滤液中分离出能引起肉瘤的因子，继而又把该因子称之为劳氏肉瘤病毒。它(们)是属于动物病毒中的单链RNA病毒类。主要用于对癌和疫苗的研究上。
CAS号：
性质：又称Rous肉瘤病毒。美国病理学家劳斯(F. P. Rous)在1910年发现把禽类的恶性肉瘤的组织液经伯克菲尔德(素烧)滤筒(Berkefeld filter，一种硅藻土滤器)滤出液接种于另外一些正常鸡，结果发现这些正常鸡也生长出具有与病禽肉瘤一样的恶性肿瘤，并从滤液中分离出能引起肉瘤的因子，继而又把该因子称之为劳氏肉瘤病毒。它(们)是属于动物病毒中的单链RNA病毒类。主要用于对癌和疫苗的研究上。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条