1) peripheral lymphocyte proliferating reaction
外周血淋巴细胞增殖反应
2) lymphocyte proliferation reaction
淋巴细胞增殖反应
1.
Methods:Effects of AI and/or EA on splenic lymphocyte proliferation reaction,Con A induced IL-2 production,dynamic changes of nature killer (NK) cell activity before and on the 1st,2nd,3rd,5th,7th day after surgical trauma were observed.
方法:观察大鼠脾脏淋巴细胞增殖反应、Con A 诱导的白细胞介素2(IL-2)水平和自然杀伤(NK)细胞活性在创伤手术前和手术后1、2、3、5、7天的动态变化,以及黄芪注射液和/或电针对以上3者的影响。
2.
Methods: Different concentrations of butanol and water extracts of RBS were added to mouse spleen cell cultures supplemented with Con A; Lymphocyte proliferation reaction was detected by MTT method; IL-2 and TNF-βsecreting level were measured .
方法:向小鼠脾细胞加入Con A和南柴胡的水与正丁醇提取物进行培养,以MTT法检测淋巴细胞增殖反应,用ELISA法测定IL-2与TNF-β分泌水平。
3) Lymphocyte proliferation response
淋巴细胞增殖反应
1.
Methods: The lymphocyte proliferation response, T lymphocyte subpopulations and IL 2 production were measured after intraperitoneal injection of BSP in normal and immunosuppressed mice, and the activity of IL 1 and TNF secreted by mouse peritoneal macrophages were detected after BSP supplementation in vitro.
方法 : 用环磷酰胺造成免疫功能低下小鼠模型 ,通过腹腔注射观察 BSP对正常和免疫低下小鼠淋巴细胞增殖反应、T细胞亚群及白介素 - 2 ( IL- 2 )产生的影响 ,并在体外观察 BSP对小鼠腹腔巨噬细胞分泌白介素 - 1 ( IL- 1 )和肿瘤坏死因子 ( TNF)的影响。
4) lymphocyte proliferation
淋巴细胞增殖反应
1.
Effects of cyclosporin A on CD28/CTLA4 and CD40/CD40L co-stimulated lymphocyte proliferation;
CD28、CD40通路共刺激对淋巴细胞增殖反应的影响及CsA的抑制作用
5) lymphocyte proliferating
淋巴细胞增殖反应
1.
Methods: Different doses of LP were givent to stomach of normal or immunological suppression mice for 7 days and the effects of LP on lymphocyte proliferating, cellulotoxic effect of macrophagc, and level of NO were determined.
方法:LP不同剂量给正常或免疫抑制小鼠灌胃7 d,观察LP对小鼠淋巴细胞增殖反应,巨噬细胞细胞毒效应以及巨噬细胞和脾细胞产生NO的影响。
6) lymphocyte proliferative responses
淋巴细胞增殖反应
1.
By using the experimental model of mouse S180 ascites sarcoma, the effects of He-Ne laser irradiation combined with the traditional antitumor medicine of cyclophosphamide on the induced IL-2 activity and T lymphocyte proliferative responses of splenic T lymphocyte in S180 ascites sarcoma bearing mice were explored systematically.
00J/cm23种剂量氦氖激光照射荷瘤鼠哈德腺,同时联合应用化疗药物环磷酰胺,对S180荷瘤鼠脾脏淋巴细胞IL-2诱生活性和淋巴细胞增殖反应的影响作了系统地研究。
补充资料:人体外周血淋巴细胞培养
人体外周血淋巴细胞培养
诊法。为 检查患者染色体最简便的细胞培养技术。先于无菌条件下制备RPMI1640 或TC199等常用培养基,加适量小牛血清和PHA(植物血球凝集 素),夏季操作时最好加适量青、链霉素,调pH值为7.0~7.2左右,4℃条件下保存6个月 。检测时,以500IU/ml肝素湿润注射器,抽取患者静脉血1.5~2ml(或取耳垂、指尖或足 跟等处微量血),每份培养基(5ml)内种血0.2~0.3ml,37℃温箱内静置培养72小时,收获 前3~4小时加秋水仙素(或秋水仙胺),最终浓度为0.02μg/ml,使细胞分裂终止于中期。 取出培养物行离心、低渗和固定等一系列细胞学操作获得染色体标本,以Giemsa染色后镜检 ,可作染色体标本的普通分析。现各实验室一般将染色体行G显带或必要的其他多种显带, 以较精确地对染色体标本进行分析。
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参考词条