1) ELISA of bovine TB
牛结核ELISA
2) bovine tuberculosis
牛结核
1.
Detection of bovine tuberculosis by ESAT-6-ELISA and PPD-ELISA:A comparative study;
ESAT-6及PPD ELISA检测疫区和非疫区牛结核病的比较研究
2.
The detecting method, which overcome the difficuhy of gaining the bovine tuberculosis-detecting specimen and have a high sensitivity and specifity, can be used for early diagnosing the tuberculosis of cow breast and detecting the dairy products safety.
本研究建立了一种实时荧光PCR检测奶牛乳液中结核分支杆菌复合群的技术,克服了以往牛结核PCR检测样品取样难的缺陷,具有检测灵敏度高、特异性强等优点,可用于奶牛乳房结核的早期诊断以及乳品安全生产检测。
3.
To develop a new method to rapidly detect antibodies against Mycobacterium bovis for diagnosis of bovine tuberculosis(bTB).
为了建立一种快速检测牛分支杆菌抗体的新方法用于诊断牛结核病,利用胶体金免疫层析技术原理,用原核诱导表达的牛分支杆菌抗原蛋白MPB83和MPB70分别作为胶体金标记抗原和检测线上的捕获抗原,制备牛结核抗体检测试纸条。
3) bovine tuberculosis
牛结核病
1.
Application of the specific tetra-valent fusion protein of Mycobacterium bovis in the diagnosis for bovine tuberculosis;
牛分枝杆菌特异性四联融合蛋白在牛结核病诊断中的临床应用
2.
Quick detection of bovine tuberculosis by fluorescent quantitative PCR and its preliminary application;
牛结核病荧光定量PCR快速检测方法的建立及初步应用
3.
Development of a nested PCR for rapid detection of bovine tuberculosis;
牛结核病巢式PCR快速检测方法的建立
4) Bovine PPD
牛结核菌素
6) bovine paratuberculosis
牛副结核病
1.
In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10.
为了建立一种有效的牛副结核病间接ELISA方法,本研究设计了两对引物,从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、,采用重叠延伸剪接技术(splice by overlapping extension,SOE)获得融合基因map0862-2154c。
补充资料:牛副结核病
由副结核分枝杆菌(Mycobacterium paratuberculosis,又名约内氏分枝杆菌)引起的牛慢性增生性肠炎。也称约内氏病。呈世界性分布,中国也有发生。2~3岁的牛最易得病,表现为卡他性肠炎,长期顽固性腹泻,极度消瘦。特征性病变为肠粘膜高度增厚并形成皱襞,在皱襞褶中藏有大量成堆的抗酸性杆菌,但无结核或溃疡。绵羊和山羊偶也可被感染。用副结核菌素或禽结核菌素进行皮肤变态反应试验或在实验室作补体结合试验,有助于检出病牛。化学疗法无甚效果。现已有用死菌苗作预防的报道。
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