1) Electroporation
电激法
1.
MACC/C95),the microalgal cells were transformed with a plasmid containing the phytase gene under the control of CaMV35S promoters and the neomycin phosphotransferase(nptⅡ) gene as a selectable gene by the method of electroporation,and the strains of transformation were selected out by the plate of G418,and then all of transformation strains were checked by the PCR and Sou.
为建立海水小球藻外源基因转化系统,使外源植酸酶基因在海水小球藻细胞内获得稳定表达,利用电激法将植酸酶基因与海水小球藻细胞共转化;利用含G418的抗性平板筛选抗性藻株,并对所获得的抗性藻株依次进行PCR检测、Southern杂交分析和植酸酶活性与性质的检测。
2.
Transfection of Plant Protoplasts with Tobacco Mosaic Virus RNA by Using Electroporation, PEC and Cationic Liposome-ediated Methods;
应用电激法和聚乙二醇法以及脂质体协调的上述两种方法对烟草和青菜原生质体进行烟草花叶病毒TMV-RNA的导入试验,并应用酶标免疫技术、电镜观察、半叶接种和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)等方法对在原生质体中增殖的TMV进行鉴定。
2) induced polarization
激电法
1.
Algorithm of forward and inversion of dual-frequency induced polarization method on elliptical sphere;
椭球体上双频激电法的正演与反演算法
2.
In this paper,a method of fast numerical simulation is presented in 3-D induced polarization.
将一种快速数值模拟方法用于激电法正演模拟中,利用在低电阻率差情况下,积分方程法模拟时阻抗矩阵的非对角线项可忽略,而只需计算矩阵主对角值这一关键点。
3) IP method
激电法
1.
Application of the TEM and IP method to the prospecting in the Hahaigang Cu polymetallic ore area of Mozhugongka County,Tibet;
瞬变电磁法和激电法在西藏墨竹工卡哈海岗铜多金属矿区找矿中的应用
2.
:With some practical examples, this paper has summed up the effectiveness of IP method in the exploration of underground water and the watersearching regularity in the army water supply.
通过几个实例,总结了在部队供水中,激电法在地下水探测中的应用效果及找水规律。
4) induced polarization method
激电法
1.
The application of induced polarization method to oil and gas exploration in western Sichuan area;
激电法在川西地区油气勘探中的应用
2.
According to theoretical basis of previous researches and summarization of geophysical prerequisite for oil prospecting with induced polarization method, the author carried out test as well as production work of this method.
本文依据前人的理论基础,在总结分析激电法找油的地球物理前提条件下,开展了该方法的试验兼生产工作,取得了令人满意的效果,并进一步证实了激电法找油的异常模式。
6) electrization
[i,lektrai'zeiʃən]
电激法,电疗法
补充资料:电激法
分子式:
CAS号:
性质:将外源DNA导入细胞内一种途径。指在脉冲电场(电压1~12kV)作用下使DNA分子进入目的细胞,电激转化率较高,可达109~1010细胞/μgDNA。此法几乎可用于所有细胞的转化。也有报道电压在250~750V/cm之间,电脉冲时间以20~100ms之间最佳。
CAS号:
性质:将外源DNA导入细胞内一种途径。指在脉冲电场(电压1~12kV)作用下使DNA分子进入目的细胞,电激转化率较高,可达109~1010细胞/μgDNA。此法几乎可用于所有细胞的转化。也有报道电压在250~750V/cm之间,电脉冲时间以20~100ms之间最佳。
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