1) Monosomic addition lines of apomictic suger beet
甜菜单体附加系
2) Monosomic addition line M14 of sugar beet
甜菜单体附加系M14
3) Sugar Beet M14
甜菜M14单体附加系
1.
Using the fresh leaves,leafstalks of plants and leaves,eustipes of aseptic seedlings of Sugar Beet M14 as the explants,which were inoculated on MS medium supplemented with different hormone and nourishing substances,such as 6-BA,NAA,IBA etc.
本研究以甜菜M14单体附加系的叶片、叶柄、无菌苗的茎节、叶片等为外植体,接种在附加不同浓度6-BA、NAA、IBA等激素的基本培养基上,通过愈伤组织和不定芽的诱导、继代、生根,从不定芽这种途径确立一套‘甜菜M14单体附加系 组织培养快速繁殖技术体系,研究了影响M14组织培养和植株再生的主要因素,着重探讨适合‘甜菜M14单体附加系 的最佳培养基配方及培养程序。
5) monosomic addition lines
单体附加系
1.
In order to clone apomixis related genes of M14 bud of the monosomic addition lines of Beta vulgaris,the cDNA library of M14 bud was constructed with a quick method.
为了克隆甜菜单体附加系M14无融合生殖相关基因 ,采用cDNA文库快速构建法制备了M14花蕾cDNA文库。
6) Monosomic addition line
单体附加系
1.
The regenerated plants of monosomic addition line M14 of sugar beet wasacquired from unpollinated ovules, and then genetic relationship between theregenerated plants and the parental plants was analyzed by performing AFLP, thus themode of reproduction of M14, obligate apomixis or facultative, was identified by meansof molecular biology.
利用甜菜单体附加系M14品系未授粉胚珠培养获得再生植株，采用AFLP技术分析再生植株与亲本植株之间的亲缘关系，从而用分子生物学手段鉴定M14品系的生殖方式是专性无融合生殖还是兼性无融合生殖。
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