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1)  telomerase catalytic subunit core promoter
hTERT核心启动子
1.
Vascular endothelial growth factor enhancer and telomerase catalytic subunit core promoter purpose fragments were amplified by genome from human liver cancer cell.
以人肝癌细胞基因组DNA为模板,应用PCR方法扩增VEGF增强子和hTERT核心启动子序列,该序列与NCBI数据库参考序列相比,同源性达到99%以上。
2)  hTERT gene core promoter
hTERT基因核心启动子
1.
Construction of TRAIL gene eukaryotic expression vector modulated by hTERT gene core promoter and its effect on apoptosis of ovarian cancer cells;
hTERT基因核心启动子调控的TRAIL基因表达载体的构建及对卵巢癌细胞凋亡的影响
3)  hTERT promoter
hTERT启动子
1.
Changes of biological character of SKOV3 cells transfected with HSV-tk gene under the control of hTERT promoter;
hTERT启动子调控的HSV-tk基因逆转录病毒载体的构建及SKOV3转染细胞的生物学特性观察
2.
Aim: To investigate the effect of ganciclovir and topotecan on SKOV3 cell transfected HSV tk under the control of hTERT promoter.
目的 :探讨更昔洛韦 (GCV)与拓扑替康 (TPT)单独或联合应用对携带有hTERT启动子调控的HSV tk基因的SKOV3细胞生长的影响。
3.
METHODS: The hTERT promoter and TK gene were cloned, andwhich was connected to the PGL3 carrier after enzyme digestion, toconstruct the hTERTp/PGL3、TK/PGL3、hTERTp/TK/PGL3 recombinantcarriers, and then to construct the CMV/TK/PGL3 recombinant carrierswith the similar method and regard CMV a.
方法:克隆hTERT启动子及TK基因,酶切后连接到pGL3载体上,构建hTERTp/pGL3,TK/pGL3,hTERTp/TK/pGL3重组载体。
4)  the promotor of h-HTERT
h-HTERT启动子
5)  hTERT promotor
hTERT基因启动子
1.
Respecting that natural hTERT promotor is not enough excellent in transcription activity and specificity, we design our research to obtain a high specificity and transcription activity hTERT promotor that expressed in telomerase.
然而天然hTERT启动子在转录活性与特异性方面不够理想,为获取一种在端粒酶表达阳性细胞中具有高特异性和较强转录活性的hTERT基因启动子,我们以天然hTERT基因编码序列启始子ATG上游270bp碱基序列为基本框架结构,进行启动子重组设计,在其下游接入四个E-box(CACGTG)重复序列结构,用化学合成法将该序列进行人工合成并亚克隆入PUC57克隆载体中;构建表达增强型绿色荧光蛋白(enhanced green fluorescent protein,eGFP)报告基因的慢病毒载体,并用重组的hTERT基因启动子片段取代慢病毒载体eGFP上游的巨细胞病毒(cytomegalovirus,CMV)启动子;将新得到的重组慢病毒载体用脂质体介导法转入端粒酶阳性的人胚肾上皮肿瘤细胞(293FT)、肝癌细胞(HepGⅡ。
6)  core promoter
核心启动子
1.
Construction of recombinant eukaryotic expression plasmid containing hepatitis B virus genome with partial deletion of the core promoter;
HBV核心启动子部分缺失重组质粒的构建
2.
The core promoter (CP), which locates in the vicinity of the transcription start site (TSS) and consists of about 100 nucleotides, is a functional region responsible for the binding to the transcription factor (TF) and the regulation of transcription by working with other cis-acting elements such as enhancers and silencers.
核心启动子(core promoter,CP)是位于转录起始位点附近由100个左右核苷酸所构成的功能区域,负责与转录因子结合同其它顺式作用元件如增强子、沉默子一起对转录进行调控。
补充资料:启动子
分子式:
CAS号:

性质:(一)在DNA分子上,可被RNA聚合酶识别并与之结合的一段序列,位于结构基因的上游,在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子,以期取得更多的表达量。(二)一种致癌因素,在癌变过程的第二阶段或多阶段机制中造成第二阶段(促进阶段);一种辅助致癌物。

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