2) EST database
EST数据库
1.
In silico cloning based on EST sequence is a new method of gene cloning,using Arabidopsis thaliana homogentisate phytyltransferase cDNA sequence as a query probe to blast Glycine max EST database,a soybean HPT gene cDNA of 1 777 bp was obtained(GeneBank Accession,AY956421).
采用基于EST的电子克隆方法,以拟南芥(Arabidopsis thaliana)的尿黑酸叶绿基转移酶基因(Homogentisate phytyltransferase,HPT)cDNA序列为信息探针,对大豆的EST数据库进行同源检索筛选,获得了1 777 bp长大豆HPT基因的cDNA序列(GenBank登录号为:AY956421),经RT-PCR扩增、分子克隆和序列分析验证,表明与电子克隆序列一致;该基因具有完整的开放阅读框架(ORF,173～1 408 bp),推测编码411个氨基酸的蛋白。
2.
A query probe from the cDNA sequence of Arabidopsis thaliana pyridoxine biosynthesis protein was designed to blast Glycine max EST database according to the relative conservation of homologous genes and a soybean PDX gene cDNA of 1 280 bp was obtained(GenBank Accession,DQ139265).
根据物种间同源基因相对保守的特点,以拟南芥(Arabidopsis thaliana)吡哆醇生物合成蛋白cDNA序列为信息探针,对大豆(Glycine max)EST数据库进行同源搜索和序列拼接,获得了1 280 bp长的大豆吡哆醇生物合成蛋白的基因序列(GenBank登陆号为DQ139265)。
3.
A novel honeybee adenine nucleotide translocator gene (Am ant) cDNA (GenBank Accession, AY332626) was cloned by blasting the honeybee EST database with the fruit fly homologous ant gene cDNA sequence information as a querying probe.
以果蝇腺苷酸转移载体基因cDNA序列为信息探针,对蜜蜂EST数据库进行同源检索筛选,克隆了蜜蜂腺苷酸转移载体基因(Am ant)的cDNA序列(GenBank登记号为AY332626),该基因全长1 251bp。
4) Genome and EST database
基因组和EST数据库
5) Endoscopic sphincterotomy
EST
1.
Objective To compare the therapeutic efficacy of endoscopic sphincterotomy(EST)with that of routine treatment in dealing with actue bilogenic pancreatitis(ABP).
目的比较早期EST治疗ABP与常规治疗结果的差异。
6) Expressed Sequence Tag
EST
1.
Construction of Intestinal cDNA Library and Analysis of Some Expressed Sequence Tags Sequencing of Ctenopharyngodon Idellus;
草鱼肠道组织cDNA文库构建及部分ESTs分析
2.
EST-SSR marker is a new kind of SSR markers which is developed from the ESTs(expressed sequence tags).
小麦EST-SSR s是从小麦ESTs序列中开发的一种新型SSR标记。
3.
Seven thousand seven hundred and fourteen contigs were obtained by assembling 13 007 expressed sequence tags (ESTs) of Chinese cabbage (Brassica campestris L.
ssppekinensis)13007个EST序列进行拼接共得到7714个片段重叠群(contig),其中10。
补充资料：数据通信网(见数据通信)
数据通信网(见数据通信)
data communication network
shu)u tongxinwang数据通信网(datac。mmunicati。nne饰ork)见数据通信。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条