CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4?8-cell embryos.

Rana temporaria chensinesis David oviductal epithelial cell was primary cultured and subcultured in vitro, these cells were identified epithelial cell by using immuno-reactivity.

The oviductal epithelial cell system can prolong the time of embryo culturing in vitro and amplify the number of embryo able to reach the blastocyst stage.

The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P<0.

Effects of preimplantation embryos on expressions of DNA methyltransferase 1 in mouse oviduct epithelial cells;

The main purpose of dissociation and culture of oviduct epithelial cells and follicle granulose cells was to break early embryo development block in vitro fertilization,to establish co-culture system in favor of early embryo development in vitro in yak.

The oviduct epithelial cells and endometrial cells were isolated and cultured in vitro,then inoculated into 4-well plate.

For simulating the natural environment in vivo, we established the co-culture systems of human oviductal epithelial cells and added EGF to the in vitro cultured mouse embryoes, and determined the effe.