Northern-blot,Northern-blot,在线英语词典,英文翻译,专业英语

1) Northern-blot

Northern-blot

The expression character of OsEXP3 and OsEXP5 genes in the roots was studied through the method of Northern-blot.

本研究根据其报道的mRNA序列设计引物,利用RT-PCR技术从4种旱稻根系中克隆了这2个基因的部分编码框序列,证明OsEXP3与OsEXP5基因在旱稻的根系中表达;通过Northern-blot技术对该表达特性进行了研究。

2) northern blot

Northern blot

3) Northern blot

Northernblot

To further understand the biogenesis and functions of miRNA, and to quantify miRNA expression levels, the expression of miR-28 in B lymphoma cell lines was detected by solution hybridization and Northern blot, and their detected results were compared.

为了进一步明确miRNA的加工机制及功能和定量研究miRNA的表达水平,应用液相杂交和Northernblot两种方法检测miR-28在B细胞淋巴瘤细胞系中的表达并进行了比较。

4) Northern blot

Northern印迹杂交

Methods The probes corresponding to the genes were amplified by RT PCR from mouse kidney, and then Northern blotting was performed on mouse multiple tissues membrane.

方法RT-PCR方法扩增WNK1和WNK4基因片段作为探针,在小鼠多组织膜上进行Northern印迹杂交。

Method The specific fragments of the two transcripts were amplified by RT-PCR and identified by Northern blot.

方法　RT PCR扩增两个转录本的特异性片段 ,Northern印迹杂交证实片段特异性后 ,将片段克隆入pGEM T载体中 ,体外转录同位素标记的正义和反义RNA探针 ,在小鼠肾脏组织石蜡切片上进行原位杂交检测。

The gene expressions of matix metalloproteinase\|2(MMP\|2) and membrane type matrix metalloproteinase (MT\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\|2 was exam.

方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。

5) Northern blot

Northern印迹

MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transc.

对其中的运动相关蛋白 (motility- related protein- 1,MRP- 1/CD9)基因 ,用 Northern印迹及逆转录 -聚合酶链反应技术分析在正常及不同病理状态下该基因表达的情况。

Among these techniques, Northern blot, ribonuclease protection assay(RPA), real-time quantitative polymerase chain reaction(RQ-PCR), which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法,即Northern印迹、RNase保护试验和实时定量PCR技术,特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

9 fold more in the resistant strain than in the susceptible strain, by reverse Northern blot and Northern blot assays.

用逆Northern印迹和Northern印迹法进一步鉴定淡色库蚊对溴氰菊酯抗药性和敏感性品系胰蛋白酶的表达差异 ,结果显示 ,胰蛋白酶基因在抗药性品系中的表达量分别是敏感性品系的 4。

6) Northern blot

Northern杂交

HSP72 mRNA and HSP70 expression in heart and vessel tissues of both simulated weightless and control rats exposed to heat stress (ambient temperature, Ta=43℃) and recovered at Ta of 25℃ for 1 h (CON H 1, SUS H 1) or 2 h (CON H 2, SUS H 2) were analyzed using Northern blot and Western blot.

用Northern杂交与Western印迹分析检测 4周模拟失重大鼠热应激后并在室温下恢复 1h (SUS H1)或 2h (SUS H2 )心肌、血管组织HSP70表达的变化。

Northern blotting showsd that 7 of 8 clones selected randomly were specially expressed in fertile anther.

随机选取 8个克隆进行Northern杂交分析 ,结果其中 7个克隆在可育花药和不育花药之间的表达存在差异。

Northern blot suggested that the transcription of Fe(II)-transporter gene was induced and strengthened by iron stress in roots; The transcription of Nramp gene was induced in both roots and leaves and strengthened in lea.

Northern杂交结果表明 ,在根中 ,irt的转录受缺铁胁迫诱导 ,并随缺铁胁迫处理天数的增加而加强 ;在根和叶中 ,Nramp基因均能得以转录且叶中的转录受缺铁胁迫诱导而加

参考词条

Northern blot Northern blot Northern blot northern blot Northern blot Northern blot Northern blot Northern blot Northern blot Northern Blot analysis Reverse northern blot northern blot analysis reverse northern blot reverse northern blot reverse northern blot Northern blot analysis WS-PolicyAttachment 商业经营者

补充资料：Northern blotting

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性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引 Northern blot Northern blot

1) Northern-blot Northern-blot 1. The expression character of OsEXP3 and OsEXP5 genes in the roots was studied through the method of Northern-blot. 本研究根据其报道的mRNA序列设计引物,利用RT-PCR技术从4种旱稻根系中克隆了这2个基因的部分编码框序列,证明OsEXP3与OsEXP5基因在旱稻的根系中表达;通过Northern-blot技术对该表达特性进行了研究。 2) northern blot Northern blot 3) Northern blot Northernblot 1. To further understand the biogenesis and functions of miRNA, and to quantify miRNA expression levels, the expression of miR-28 in B lymphoma cell lines was detected by solution hybridization and Northern blot, and their detected results were compared. 为了进一步明确miRNA的加工机制及功能和定量研究miRNA的表达水平,应用液相杂交和Northernblot两种方法检测miR-28在B细胞淋巴瘤细胞系中的表达并进行了比较。 4) Northern blot Northern印迹杂交 1. Methods The probes corresponding to the genes were amplified by RT PCR from mouse kidney, and then Northern blotting was performed on mouse multiple tissues membrane. 方法RT-PCR方法扩增WNK1和WNK4基因片段作为探针,在小鼠多组织膜上进行Northern印迹杂交。 2. Method The specific fragments of the two transcripts were amplified by RT-PCR and identified by Northern blot. 方法　RT PCR扩增两个转录本的特异性片段 ,Northern印迹杂交证实片段特异性后 ,将片段克隆入pGEM T载体中 ,体外转录同位素标记的正义和反义RNA探针 ,在小鼠肾脏组织石蜡切片上进行原位杂交检测。 3. The gene expressions of matix metalloproteinase\\|2(MMP\\|2) and membrane type matrix metalloproteinase (MT\\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\\|2 was exam. 方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。 5) Northern blot Northern印迹 1. MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transc. 对其中的运动相关蛋白 (motility- related protein- 1,MRP- 1/CD9)基因 ,用 Northern印迹及逆转录 -聚合酶链反应技术分析在正常及不同病理状态下该基因表达的情况。 2. Among these techniques, Northern blot, ribonuclease protection assay(RPA), real-time quantitative polymerase chain reaction(RQ-PCR), which are based on differentially screening and hybridization technology, have been applied most frequently. 本文概述了用于检测mRNA丰度的常规分析方法,即Northern印迹、RNase保护试验和实时定量PCR技术,特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。 3. 9 fold more in the resistant strain than in the susceptible strain, by reverse Northern blot and Northern blot assays. 用逆Northern印迹和Northern印迹法进一步鉴定淡色库蚊对溴氰菊酯抗药性和敏感性品系胰蛋白酶的表达差异 ,结果显示 ,胰蛋白酶基因在抗药性品系中的表达量分别是敏感性品系的 4。 6) Northern blot Northern杂交 1. HSP72 mRNA and HSP70 expression in heart and vessel tissues of both simulated weightless and control rats exposed to heat stress (ambient temperature, Ta=43℃) and recovered at Ta of 25℃ for 1 h (CON H 1, SUS H 1) or 2 h (CON H 2, SUS H 2) were analyzed using Northern blot and Western blot. 用Northern杂交与Western印迹分析检测 4周模拟失重大鼠热应激后并在室温下恢复 1h (SUS H1)或 2h (SUS H2 )心肌、血管组织HSP70表达的变化。 2. Northern blotting showsd that 7 of 8 clones selected randomly were specially expressed in fertile anther. 随机选取 8个克隆进行Northern杂交分析 ,结果其中 7个克隆在可育花药和不育花药之间的表达存在差异。 3. Northern blot suggested that the transcription of Fe(II)-transporter gene was induced and strengthened by iron stress in roots; The transcription of Nramp gene was induced in both roots and leaves and strengthened in lea. Northern杂交结果表明 ,在根中 ,irt的转录受缺铁胁迫诱导 ,并随缺铁胁迫处理天数的增加而加强 ;在根和叶中 ,Nramp基因均能得以转录且叶中的转录受缺铁胁迫诱导而加参考词条 Northern blot Northern blot Northern blot northern blot Northern blot Northern blot Northern blot Northern blot Northern blot Northern Blot analysis Reverse northern blot northern blot analysis reverse northern blot reverse northern blot reverse northern blot Northern blot analysis WS-PolicyAttachment 商业经营者补充资料：Northern blotting 分子式： CAS号：性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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