1) swine pepsinogen A gene
猪胃蛋白酶原A基因
1.
24 kb segment of swine pepsinogen A gene cDNA by RT-PCR.
根据GenBank中发表的猪胃蛋白酶原A(pepsinogen)基因序列设计引物,采用RT-PCR技术,成功获得了猪胃蛋白酶原A基因,该基因全长1 240 bp,将该基因克隆到表达载体pPIC3。
2) pepsinogen A
猪胃蛋白酶原A
1.
The Hubei White swine pepsinogen A cDNA was cloned by RT-PCR,which were 1 227bp in length(submitted in Genebank,the accession number is EF108301.
根据Genebank公布的J04601猪胃蛋白酶原A序列,进行了引物的设计和筛选,通过RT-PCR获得了湖北白猪的胃蛋白酶原A的cDNA序列全长,共1227bp(已提交Genebank,登录号EF108301),其具有一个长度为1158bp的读码框,编码385个氨基酸,其中包含15个氨基酸组成的信号肽,成熟的胃蛋白酶原A由370个氨基酸残基组成。
2.
The pepsinogen A from the Hubei swine was inset the pPIC3.
利用已有的猪胃蛋白酶原A cDNA,构建毕赤酵母pPIC3。
3.
Based on the cloning and sequence analysis of pepsinogen A from the Hubei swine,constructed plasmid of pPIC3.
利用已有的猪胃蛋白酶原AcDNA,构建毕赤酵母pPIC3。
3) porcine pepsinogen
猪胃蛋白酶原
1.
High-level expression and purification of porcine pepsinogen A in pichia pastoris;
猪胃蛋白酶原A在毕赤酵母中的高效表达及其分离纯化
4) Pepsinogen C gene
胃蛋白酶原C基因
1.
A study of polymorphism of pepsinogen C gene among the personswith bad life habit.;
具不良生活习惯者胃蛋白酶原C基因多态性的研究
5) pepsin from porcine gastric mucosa
猪胃蛋白酶
1.
Objective;To optimize the extraction technology of pepsin from porcine gastric mucosa with hydrochloric acid.
目的:优化猪胃蛋白酶的酸法提取工艺。
6) pepsinogens
胃蛋白酶原
1.
In this work, we reported a method for the purification of pepsinogens from human gastric mucosa with high pressure gel filtration chromatography(HPLC) and medium pressure anion exchange chromatography(MPLC).
首次应用高压凝胶过滤色谱和中压阴离子交换色谱提纯胃蛋白酶原，得到抗原ＰＧⅠ和ＰＧⅡ，一次提纯仅需５０ｍｉｎ。
2.
Four pepsinogens have been purified from sea bream and mandarin fish, respectively by ammonium sulfate fractionation, DEAE-Sephacel chromatography and Sephacryl S-200 gel-filtration for the first time and their characteristics be studied.
海水黄鳍鲷和淡水鳜鱼胃蛋白酶原的纯化和酶的性质研究,迄今为止,国内外未见报道。
3.
Four pepsinogens were isolated from rice field eel and three pepsiogens were purified to homogeneity from the stomach of European eel by ammonium sulfate fractionation, column chromatographies on anion exchange and gel-filtration.
本研究以淡水黄鳝和欧洲鳗鲡为材料,通过硫酸铵盐析、DEAE-Sephacel离子交换层析以及Sephacryl S-200、Superdex 75凝胶过滤层析等方法分别从黄鳝胃内纯化得到四种胃蛋白酶原,从欧洲鳗鲡胃内纯化得到三种胃蛋白酶原,并对相应酶的性质进行了比较分析。
补充资料：血浆胃蛋白酶原
血浆胃蛋白酶原
*胃蛋白酶原进入血液循环。在消化性溃疡患者血浆中胃蛋白酶原浓度增高；萎缩性胃炎、恶性贫血、胃切除术后其浓度则降低。测定血浆胃蛋白酶原对诊断上述疾病具有一定参考价值。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条