1) laser microbeam puncture
激光微束穿刺法
1.
By means of laser microbeam puncture,plasmid pBUBA containing insect-resistant genes of API-A、API-B and selective marker gene of NPT-Ⅱwere transferred into Anthurium andraeanum Lind.
通过激光微束穿刺法将构建的含抗虫基因API-A、API-B和选择性标记基因NPT-Ⅱ的质粒pBUBA导入红掌细胞中,通过卡那霉素抗性筛选和聚合酶链式反应(PCR)技术分析表明,抗卡那霉素的绿苗中大部分为阳性植株。
2) laser microbeam puncture
激光微束穿刺
1.
Acquisition of insect-resistant transgenic Brassica napus and Its Progeny by laser microbeam puncture;
激光微束穿刺法获得抗虫转基因油菜及其后代的研究
2.
The PAP cDNA controlled by a wound-inducible promoter was introduced into Brassica napus via laser microbeam puncture, and plantlets resistant to Gentamycin were obtained.
采用激光微束穿刺法将损伤诱导型启动子控制之下的商陆抗病毒蛋白（PAP）CDNA导入了甘蓝型油菜（BrassicanapusL。
3) Laser microbeam puncture technique (LMPT)
微束激光穿刺技术
4) laser micro-beam
激光微束
1.
The overall design of the laser micro-beam system is presented with the design of critical components for the required gradient force field for forming the optical tweezers and the energy needed to form the optical scissors.
提出了构建一种用于细胞非接触操作的激光微束系统。
2.
The effects of laser micro-beam on optical trapping force are introduced.
在几何光学近似下,利用光线追踪法分析了激光微束作用于微粒的作用力,数值计算中考虑了激光微束的具体结构对光阱力的影响。
3.
This article reviews the development and outcome of the cell fusion of fish,The methods of cell fusion of fish include fusion induced by virus,PEG,electric field pulse and laser micro-beam.
概述了鱼类细胞融合研究的发展和主要成果 ,鱼类细胞融合技术主要包括病毒法、PEG法、电融合法、激光微束法 。
5) laser microbeam
激光微束
1.
Us ing this focusing system, the laser microbeam instrument has got the focal spot size of less than 0.
用这个激光聚焦系统，激光微束仪能获得直径小于０。
2.
The recent advances about laser microbeam in plant transformation of exogenous gene,key techniques and applications are introduced.
本文概述了近年来激光微束在植物外源基因转化上的最新进展,激光微束转化法的关键技术及应用。
3.
The recent advances about laser microbeam applications in chromosome microdissection and isolation, molecular cell biology, cell wall removing, protoplast fusion are summarized.
本文概述了近年来激光微束在染色体微切割和微分离、分子细胞生物学、去除细胞壁、诱导原生质体融合等方面的应用 ,并对其今后的应用前景作了展
6) laser microbeam
微束激光
1.
Regard genome DNA of rice as the template,have cloned the upper sequence of the gluten genetic Gt1 of rice by PCR method,and construct it on the plant expression carriers with green fluorogenic albumen,shell with laser microbeam method receptor to transform maize organize,and start the function of son through the method that express instantly.
以水稻基因组DNA为模板,用PCR方法克隆了水稻谷蛋白基因G t1的启动子序列,并将其构建到带有绿色荧光蛋白基因(gfp)的植物表达载体上,用微束激光穿刺法转化玉米的受体组织,通过外源基因瞬时表达的方法验证了该启动子的功能,结果表明在胚乳中有较强的表达,而在其它组织中表达很弱;证明了水稻谷蛋白基因G t1的启动子在不同物种玉米中同样具有胚乳特异表达的功能。
2.
Using cotton susceptive seed-germinating embryos as transgenic receptor,the bivalent genes pBIBGC,which includes β-1,3-glucanase gene and chitinase gene,was introduced into cotton by laser microbeam Puncture Techniques(LMPT).
研究了微束激光转化法用于棉花感受态萌动胚转化预培养的时间、高渗液对材料的处理等。
补充资料：激光微束技术
分子式：
CAS号：
性质：利用显微镜把引入显微镜的激光聚焦，可以在样品上获得直径与光波长相当的激光微束，用以对细胞进行各种加工，包括对膜、染色体、中心粒等的加工。也可用此法在膜上打孔、进行外源基因的导入，以及进行细胞融合。
CAS号：
性质：利用显微镜把引入显微镜的激光聚焦，可以在样品上获得直径与光波长相当的激光微束，用以对细胞进行各种加工，包括对膜、染色体、中心粒等的加工。也可用此法在膜上打孔、进行外源基因的导入，以及进行细胞融合。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条