1) yeast colony PCR
酵母菌落PCR
1.
Recombinants were identified by yeast colony PCR, then were induced by methanol and detected by SDS-PAGE analysis.
berezovskii)白介素-2(interleukin-2,IL-2)到酵母表达载体pPIC9K,SalⅠ酶切后转化毕赤酵母GS115,G418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,SDS-PAGE分析,结果在诱导至第6d的酵母上清浓缩液中,检测到与预测林麝IL-2分子量相接近的,分子量约为18Ku的诱导表达带;TRIzol提取表达期间的酵母总RNA,并通过RT-PCR扩增IL-2mRNA,发现表达期的重组酵母细胞中存在IL-2mRNA,而对照没有检出,表明林麝IL-2存在低水平的表达。
2.
Recombinants were identified by yeast colony PCR.
5K上,SalI线性化后转化毕赤酵母GS115,418抗性筛选高拷贝克隆,再由酵母菌落PCR鉴定;阳性克隆用甲醇诱导表达,Tricine-SDS-PAGE分析,结果在诱导第2d的酵母裂解液中检测到与预期的SMAP-29分子量接近,约3。
2) yeast type colony
酵母型菌落
3) yeast petite mutation
酵母小菌落突变
4) yeast like colony
类酵母型菌落
5) yeast
[英][ji:st] [美][jist]
酵母菌;酵母
6) colony PCR
菌落PCR
1.
False positives resulted from colony PCR in rapid identification of recombinant clones from phage antibody library;
菌落PCR快速分析抗体库重组率时的假阳性现象
2.
Selection and identification of recombinant plasm ids using colony PCR method;
菌落PCR技术在重组质粒筛选与鉴定中的应用
3.
To establish a quick, sensitive and economical PCR method for directly detecting tlh and adh genes-carrying Vibrio parahaemolyticus in environment and seafood, a colony PCR method was developed and its results were compared with those of the conventional PCR method, thus establishing a colony PCR method.
为建立一种快速、经济、敏感的PCR检测方法 ,以直接检测出环境、海产品等中的靶基因 ,通过PCR扩增已知携带tlh和tdh基因的副溶血弧菌标准菌株 ,并和常规PCR方法比较 ,初步建立了菌落PCR技术 ;再以副溶血弧菌标准菌株为阳性对照 ,以本实验室自海洋环境中分离得到的数株野生菌株为检测对象 ,采用新建立的菌落PCR以及常规PCR方法对它们进行扩增比较 ,进一步证明了菌落PCR技术检测携带相关基因菌株的可靠性 。
补充资料：大肠杆菌不同生化突变型菌落(显示不同颜色)
乔守怡提供
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条