1) T-DNA activation tagging
T-DNA激活标签
2) T-DNA tag
T-DNA标签
1.
Using calli from cotyledon and hypocotyl of pepper inbred line H8124 as explants,T-DNA tag was inserted into pepper through agrobacterium-mediated transformation.
以辣椒自交系H8124无菌苗子叶和下胚轴为外植体,采用根癌农杆菌侵染转化法,将T-DNA标签导入辣椒,以探讨影响转化效率的主要因素,包括外源植物生长调节剂浓度、农杆菌类型、外植体材料、预培养时间、侵染时间等。
3) T-DNA tagging
T-DNA标签
1.
T-DNA tagging and its applicatlon in plant functional genome research;
T-DNA标签技术及其在植物功能基因组研究中的应用
2.
The paper summarized the procedure for analyzing gene function by T-DNA tagging and the methods to clone the tagged gene of mutants gotten by transformation,which would facilitate the analysis of gene function using T-DNA tagging.
概述了应用T-DNA标签进行基因功能分析的步骤及获得突变体后标签基因的分离方法,对应用T-DNA标签进行基因功能分析研究具有一定的参考价值。
3.
Agrobacterium-mediated T-DNA tagging is an effective molecular technology in recent years.
农杆菌介导的T-DNA标签法是近年发展的一种有效的分子生物学技术。
6) Activation tagging
激活标签
1.
Due to this special characteristics of activation tagging,this method has been a powerful tool for new gene discovery and gene functional analysis.
激活标签法是新近发展起来的一种用于基因分离和鉴定的方法。
2.
Through activation tagging,we identified an early flowering mutant called scc17-D(suppressor of cry1cry2#17-Dominant).
采用激活标签的方法,在cry1cry2双突变体背景下筛选到一个早开花突变体scc17-D(suppressor of cry1cry2#17-Dominant)。
3.
Genetic transformation for cabbage was studied using hypocotyls of \'Xiaguang\' variety and Agrobacterium tumefaciems GV3101 (with activation tagging plasmid pSKI015).
以夏光甘蓝下胚轴为材料,采用含激活标签pSKI015的农杆菌GV3101进行遗传转化研究,对预培养时间、侵染时间和共培养时间等转化条件进行优化。
补充资料：DNA分子花窗(沿B―DNA轴线方向看)
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条