Establishment of pEGFP-hTERT vector and expression in type A spermatogonia of bovine;

Methods Type A spermatogonial stem cells were isolated from 9 day-old SD rat testes by discontinuous Percoll gradient centrifugation.

Construciton of pEGFP-N1-PLZF eukaryotic expression vector and its expression in spermatogonia;

Separation and purification of human testicle spermatogonia with Percoll centrifugation combined with different time of cellular adhesiveness;

Effects of mTR Antisense Oligodeoxynucleotide on Telomerase Activity and the Expression of Telomerase mTR in Type A Spermatogonia;

Isolation and purification of spermatogonium in rat;

Objective To study the mutability of ultra sodium pyrosulfite intake on ultrastructure changes and spermatogonium mice testis.

Chicken embryos from stage 15 to stage 45 were studied by means of serial section and light microscopy in order to learn the relationship between the spermatogonium and the testicular development in the early chicken embryo.

Spermatogonial-somatic cell co-culture model was used for studying the effect of follicle-stimulating hormone (FSH) and epidermal growth factor (EGF) on proliferation of spermatogonial cells of embryonic chickens.

Enhanced green fluorescent protein(EGFP) gene was transfected into chicken embryonic spermatogonial cells(CESCS) in vitro by three different methods,calcium acid phosphate,liposome(Sofast)and electroporation,to obtain the optimal transfection strategy by detecting the transfection efficiencies.