1) T4 phage
T4噬菌体
1.
Cap gene of PCV2 was amplified by PCR and cloned into the expressing plasmid pSOC,where Cap gene was fused to 3 end of T4 phage small outer capsid gene encoding SOC protein.
将PCV2衣壳蛋白片段与T4噬菌体SOC蛋白在大肠杆菌中融合表达。
2.
NP gene of AIV was amplified by PCR and cloned into the expressing plasmid pSOC, where NP gene was fused to 3′end of T4 phage small outer capsid gene encoding SOC protein.
将NP基因克隆至T4噬菌体表达质粒pSOC的SOC基因(T4噬菌体表面非结构蛋白基因)下游,构建成T4噬菌体SOC位点表达NP的表达载体pSOC-NP。
3.
The recombinant plasmid was transformed into Escherichia coli 2(E2),the host bacteria of T4 phage.
利用PCR技术,扩增去除部分序列,长度为1320bp,编码440个氨基酸的禽流感病毒(AIV)核蛋白基因片段,将其克隆至pR质粒的T4噬菌体SOC基因C末端获得重组质粒pR-np,以此重组载体转化大肠杆菌(Escherichia coli)2(E2),用溶菌酶缺陷噬菌体T4-z1感染重组E2菌,重组载体与缺陷噬菌体T4-z1基因发生同源重组,将np基因整合到噬菌体基因组中,用PCR方法筛选重组噬菌体并命名为T4-z1-np。
2) T4 bacteriophage
T4噬菌体
1.
Construction and identification of foot-and-mouth disease virus entire capsid protein precursor(P1) gene recombinant T4 bacteriophage;
口蹄疫病毒全衣壳前体(P1)重组T4噬菌体的构建和鉴定
2.
T4 bacteriophage surface display of foot-and-mouth disease virus 3 C protease;
口蹄疫病毒3C蛋白酶的T4噬菌体表面展示
3) bacteriophage T4
T4噬菌体
1.
Display of globular domain of HN protein of Newcastle disease virus on bacteriophage T4 capsid surface;
新城疫病毒HN基因与T4噬菌体SOC基因在大肠埃希氏菌中的融合表达
4) T4 bacteriophage display
T4噬菌体展示
5) Recombinant phage T4-VP
重组噬菌体 T4-VP2
6) T4 phage display
T4噬菌体展示系统
补充资料：T4噬菌体
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条