1) duplex fluorescent PCR
二重荧光PCR
2) dual real-time PCR
双重荧光PCR
1.
Development of a dual real-time PCR assay for rapid simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus;
沙门菌和副溶血性弧菌双重荧光PCR快速检测方法的建立
3) multiplex real-time PCR
多重荧光PCR
1.
Establishment and application of multiplex real-time PCR to detect Streptococcus suis serotype 2;
多重荧光PCR检测Ⅱ型猪链球菌方法的建立及应用
4) fluorescent monoplex PCR
荧光单重PCR
1.
The fluorescent monoplex PCR and doubleplex PCR were respectively developed.
以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列比对分析,设计了检测小麦印度腥黑穗病菌及黑麦草腥黑穗病菌的TaqM an MGB实时荧光PCR引物和探针,优化了反应条件,筛选出特异性探针,分别建立了小麦印度腥黑穗病菌和黑麦草腥黑穗病菌实时荧光单重PCR和实时荧光双重PCR检测方法,其中实时荧光双重PCR检测方法实现了在同一PCR管中仅用5μL的反应体系,进行1次PCR反应就能特异性检测出小麦印度腥黑穗病菌或黑麦草腥黑穗病菌。
5) fluorescent doubleplex PCR
荧光双重PCR
补充资料：二溴二硝基荧光素二钠
CAS：548-24-3
分子式：C20H8Br2N2O9·2Na
分子质量：624.06
中文名称：曙红B；4',5'-二溴-3',6'-二羟 基-2',7'-二硝基螺(异苯并呋喃-1(3H),9'-(9H)占吨)-3-酮二钠盐；伊红B；二溴二硝基荧光素二钠；伊红 B
英文名称：Eosin B；Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one,4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-,disodium salt
分子式：C20H8Br2N2O9·2Na
分子质量：624.06
中文名称：曙红B；4',5'-二溴-3',6'-二羟 基-2',7'-二硝基螺(异苯并呋喃-1(3H),9'-(9H)占吨)-3-酮二钠盐；伊红B；二溴二硝基荧光素二钠；伊红 B
英文名称：Eosin B；Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-, disodium salt
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one,4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-,disodium salt
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条