-10 consensus sequence (TATGTT) of mutational tac promoter was reverted to TATATT and TATGAT by PCR amplification method.

The PCR fragments were cloned to plasmid pKtac1(containing tac promoter)and two recombinant plasmids:pKtac1-act1(containing 564 bp total activator gene)and pKtac1-act2(containing 409 bp partial activator gene)were obtained.

A DNA Autonomous Replication Origin with Promoter Function in Yeast;

Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;

Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;

The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.

However, the most generally used consititutive CaMV 35S promoter often fail in some incidences, especially for those genes in male organgenesis and male gametogenesis.

And also it is strong recommended that the biosafe construct should have no any uncertain element and exogenous DNA sequence such as antibiotics gene,35S promoter etc.

Changes of biological character of SKOV3 cells transfected with HSV-tk gene under the control of hTERT promoter;

Aim: To investigate the effect of ganciclovir and topotecan on SKOV3 cell transfected HSV tk under the control of hTERT promoter.

METHODS: The hTERT promoter and TK gene were cloned, andwhich was connected to the PGL3 carrier after enzyme digestion, toconstruct the hTERTp/PGL3、TK/PGL3、hTERTp/TK/PGL3 recombinantcarriers, and then to construct the CMV/TK/PGL3 recombinant carrierswith the similar method and regard CMV a.

AIM: To construct the recombinant adenovirus carrying fusion suicide gene CDglyTK with the C promoter(Cp),one of the key factors in controlling Epstein-Barr virus latent gene expression,and to investigate if the Cp mediates the expression of CDglyTK in CNE1 cells and kills the cancer cells specifically.