1) ISSR
ISSR反应体系
1.
To optimize ISSR amplification system on Zantedeschia,the effect of four factors, Mg~(2+),Taq DNA Polymerase,primer and DNA,were studied.
通过不同浓度Mg~(2+)与TaqDNA聚合酶;模板DNA与引物的组合试验,确定马蹄莲属植物最佳ISSR反应体系为:每20μl中含1×PCR buffer、2。
2) ISSR reaction system
ISSR反应体系
1.
The results showed that optimum ISSR reaction system of calla lily was every 20 μL reaction system containing 1×PCR Buffer, 1.
选取白花马蹄莲和10个颜色有代表性的彩色马蹄莲栽培品种为材料,采用改进的SDS法提取叶片基因组DNA,通过正交设计研究Mg2+、Taq DNA聚合酶和模板DNA、引物的影响,确定彩色马蹄莲最佳ISSR反应体系为:每20μL中含1×PCR Buffer、1。
2.
Results A suitable ISSR reaction system was constructed with the 20 μL reaction system containing 1.
所建立的药用菊花ISSR反应体系具有标记位点清晰、反应系统稳定、检测多态性能力较强、重复性好等特点,可用于药用菊花的遗传多样性研究。
3) ISSR-PCR reaction system
ISSR-PCR反应体系
1.
In order to establish a stable ISSR-PCR reaction system of Scrophularian ningpoensis Hemsl,some compatible PCR systems were screened by orthogonal design on 5 factors(dNTPs,Taq DNA polymerase,MgCl2,primer and DNA template) and 5 levels,meanwhile the factors and reaction procedure(annealing temperature,elongate time and cyclic numbers) were optimized by single factor gradient.
为了建立稳定的玄参ISSR-PCR反应体系,本试验采用5因素(dNTPs、Taq DNA聚合酶、MgCl2、引物及模板)5水平正交设计筛选PCR体系,并利用单因子梯度进一步优化反应体系各因素浓度和扩增程序(退火温度、延伸时间和循环次数)。
2.
[Objective] The study aimed to establish the effective and stable ISSR-PCR reaction system for Manglietia grandis Hu et Cheng.
[目的]建立高效稳定的大果木莲ISSR-PCR反应体系。
4) ISSR-PCR reaction
ISSR-PCR反应
1.
The most suitable ISSR-PCR reaction system and amplified procedure for C.
以改良的CTAB法提取的寒兰(Cymbidium kanran Makino)基因组DNA为模板,通过单因子试验建立最适的寒兰的ISSR-PCR反应体系。
5) ISSR-PCR system
ISSR-PCR体系
1.
This paper analyzed the influence of the 6 factors on the reaction results,the factors included:the concentration of Mg2+,dNTP and BSA in ISSR-PCR system,as well as the dosage of the template DNA,primer and Taq DNA polymerase.
分析了ISSR-PCR体系中Mg2+、dNTP和BSA浓度,以及模板DNA、引物和TaqDNA聚合酶用量等6个因素对反应结果的影响,建立了适合于台湾水青冈ISSR-PCR扩增的反应体系:10μL PCR反应液中,含10 ng DNA,1。
6) ISSR Rreaction System Optimization
ISSR体系优化
补充资料：反应素性变态反应
反应素性变态反应
见"I型变态反应"。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条