2) competent cells
感受态细胞
1.
coli DH5a was cultured by air bath and water bath at 25℃,competent cells were freezed with 10% (V/V) glycerin-water solution and 10% (V/V) glycerin-brine solution.
方法于室温(25℃)用空气浴振荡和水浴振荡培养大肠杆菌DH5a,用10%(V/V)甘油-水溶液和10%(V/V)甘油-生理盐水溶液冻存所制感受态细胞。
2.
An efficient bacterial transformation system was described for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks.
描述了一种改良的高效细菌感受态的制备和感受态细胞的保藏及细菌的转化方法。
3.
As a stabilizer for frozen competent cells,the temperature of liquid nitro.
探讨了大肠杆菌生长状态、转化溶液浓度、保存温度、冰上放置时间对感受态细胞转化能力的影响。
3) competent cell
感受态细胞
1.
The effect of temperature on the competent cell of Escherichia coli;
温度对大肠杆菌感受态细胞的影响
2.
Objective:To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
目的:建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
3.
Factors including different mediums,growth status,transformation solutions,mixing time of competent cells and plasmid,amount of DNA,frozen and heat shock were evaluated on transformation of plasmid ProkⅡ.
研究了根癌农杆菌LBA 4404和C58C1生长的培养基类型、菌株生长状态、转化溶液、质粒与感受态细胞共放置时间、质粒加入量、液氮速冻时间及热激条件对质粒P rokⅡ转化的影响。
4) Preparation of competent cells
感受态制备
5) Recipient cell competent state
受体细胞感受态
补充资料:感受态细胞
分子式:
CAS号:
性质:(一)处于可转化状态的细菌细胞。(二)能识别抗原或合成抗体的生理状态时的细胞。(三)一部分胚胎能在特定方向中通过鉴定和分辨与诱导物起反应时的生理状态。
CAS号:
性质:(一)处于可转化状态的细菌细胞。(二)能识别抗原或合成抗体的生理状态时的细胞。(三)一部分胚胎能在特定方向中通过鉴定和分辨与诱导物起反应时的生理状态。
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参考词条