Construction of shRNA expression vector pWH1 and its function in silencing the HIF1 gene;

Construction and identification of siRNA expression vector targeting for Rab9 GTPase;

Construction of Saccharopolyspora erythraeaexpression vector pZW;

Construction and Functional Analysis of Short Interfering RNA Expression Vectors of Targeting Gene Apolipoprotein M;

Construction and application of an expression vectors with combined T7 and PR promoters for flexible induced expression of cloned DNA in E.coli;

This is clearly due to the fact that all the signals contained in lactoprotein genes have not yet been identified,and the mammary-gland expression vectors are not constructed in an appropriate manner.

Plant expressing vector construction of MAPK gene in Dunaliella salina and transformation to tobacco;

The construction of E3region expressing vector of CAV-2;

The identifications of PCR, enzymical degestion and sequencing were carried out, and the expressing vector of pG-Lfn was successfully constructed.

Construction of siRNA eukaryotic expression vectors of PEG10 gene and their apoptosis-inducing effect on transfected HepG2 cells;

To construct the eukaryotic expression vector of pCEP4-CaBP-D28k,a pair of primers was designed according to chicken calbindin-D28k(CaBP-D28k) gene sequence published in GenBank.

Construct of Gastrin-shRNA Vector and Its Effects on Gastric Cancer Cell Line BGC-823;

In order to construct a universal mammary gland expression vector, sheep beta lactoglobulin (BLG) gene promoter, 5′ and 3′ region of 5 kb and 4.

This system is derived from commercially available vector pETlla (Novagen) by 1) changing promotor T7 to SP6; 2) introducing 6xHis tag at N-terminal of open reading frame and 3) inserting a marker gene of RNA Polymerase which is induced by L-arabinose and thus to work as a protein expression control.