1) PK-15 cells
PK-15细胞
1.
In order to understand the replication kinetics of classical swine fever virus(CSFV) in in vitro cells PK-15 cells were seeded in 96-well tissues culture plates.
应用间接免疫荧光、Real-time PCR和病毒感染滴度(TCID50)测定技术,分别从病毒抗原、病毒基因组RNA复制水平和病毒感染滴度变化3个方面,研究了猪瘟病毒(CSFV)在PK-15细胞中增殖的特点,用猪瘟病毒石门株感染96孔板培养的细胞,1×102个TCID50/孔,间接免疫荧光检测结果显示感染后8h能检测到被荧光抗体染色的感染细胞,随感染时间的延长,出现荧光的细胞数量逐渐增多,在感染后72h,几乎所有细胞均能出现荧光。
2.
These recombinant plasmids were transfected into PK-15 cells and were screened by G418.
重组干扰载体经纯化后转染PK-15细胞,并进行G418抗性筛选。
3.
10 strains of them were S suis type 2 by PCR identification, strains with virulence factors were selected to explore the adhevence of them to PK-15 cells.
为研究携带不同毒力因子的猪链球菌2型对细胞侵袭作用的差异,本实验室从全国各地分离100多株猪链球菌,PCR鉴定出10株链球菌2型,其中携带毒力因子菌株为7-4[MRP+EF+]、8-3[MRP+EF-]、ZH-1[MRP-EF+],另外选择1株LN-2[MRP-EF-]与PK-15细胞进行黏附动力学试验,结果表明MPR毒力因子与菌株对PK-15细胞黏附具有密切相关性。
2) PK-15 cell
PK-15细胞
1.
The recombinant retroviruses was transfected into PK-15 cell by polybrene.
将该重组质粒与水疱性口炎病毒载体pVSV-G共转染GP2-293细胞,收获假型病毒,在Polybrene的介导下感染PK-15细胞,嘌呤霉素筛选阳性细胞克隆。
2.
The anti-PPV effect of chlorogenic acid which was extracted from Chinese herbal medicine was investigated in PK-15 cell by observing cytopathic effect(CPE).
本试验利用水煮醇提方法提取金银花的主要成分绿原酸,通过观察PK-15细胞病变效应(CPE)来评价绿原酸不同加药方式(先加药后接种病毒,先接种病毒后加药)对猪细小病毒(PPV)的体外作用效果。
3) PK-15
PK-15细胞
1.
These constructs were individually transformed into PK-15 cells by liposome.
利用质粒载体在细胞内转录并加工成siRNA的方法,设计3对针对猪瘟病毒3'-UTR不同位点的干扰片段,分别与干扰载体pGenesil-1连接,转化DH5α,筛选阳性克隆得到重组干扰质粒pGene-1,pGene-2和pGene-3,脂质体介导转染重组干扰质粒于PK-15细胞,G418抗性筛选得到转录靶向猪瘟病毒3'-UTR shRNA的PK-15细胞株,为今后应用RNAi研究猪瘟病毒3'-UTR调控病毒复制的功能以及抑制猪瘟病毒增殖的研究奠定了基础。
2.
Pocine kidney cells and PK-15 cells were infected by t.
收取PK-15细胞上清,在牛肾细胞(MDBK)上进行干扰素抗病毒活性检测,结果显示重组病毒表达的猪γ干扰素抗水泡性口炎病毒(VSV)的活性为1200IU/106cells。
4) NG108-15 cells
NG108-15细胞
1.
Level of PTEN protein in the process of tau hyperphosphorylation induced by okadaic acid in NG108-15 cells;
冈田酸诱导NG108-15细胞tau蛋白过度磷酸化过程中PTEN蛋白水平的变化
2.
Staurosporine induces apoptosis in NG108-15 cells;
星形孢菌素诱导NG108-15细胞凋亡(英文)
3.
Methods: NG108-15 cells were exposured to 5μmol/L Aβ25-35 for 24h to establish the cell model.
方法:经老化处理的Aβ25-35(终浓度为5μmol/L)与NG108-15细胞共同孵育24h建立AD细胞模型,运用中药血清药理学方法,以温胆汤改良方含药血清作用于AD细胞模型,倒置相差显微镜观察细胞形态,MTT法检测细胞活性,流式细胞术检测细胞凋亡,SABC免疫组化法检测p-JNK、p-c-jun蛋白的表达。
5) HCT-15 cell
HCT-15细胞
1.
Study on induction of apoptosis by girinimbine in HCT-15 cell in vitro;
吉九里香碱体外诱导HCT-15细胞凋亡的研究
补充资料：16-Dioxa-8,9,10,11-tetrathia-4,15-disilaoctadecane, 4,4',15,15-tretraethoxy-3
CAS：40372-72-3
分子式：C18H42O6S4Si2
分子质量：538.94
中文名称：4,4',15,15-四乙氧基-3,16-二氧杂-8,9,10,11-四硫杂-4,15-二硅杂十八烷 硅烷偶联剂G-407 双[γ-(三乙氧基硅)丙基]-四硫化物
英文名称：16-Dioxa-8,9,10,11-tetrathia-4,15-disilaoctadecane, 4,4',15,15-tretraethoxy-3
Coupling agent G-407
9,10,11-tetrathia-4,15-disilaoctadecane, 4,4,15,15-tetraethoxy-16-dioxa-8
bis[3-(triethoxysilyl)propyl]tetrasulfide
3,16-Dioxa-8,9,10,11-tetrathia-4,15-disilaoctadecane,4,4',15,15-tretraethoxy-
分子式：C18H42O6S4Si2
分子质量：538.94
中文名称：4,4',15,15-四乙氧基-3,16-二氧杂-8,9,10,11-四硫杂-4,15-二硅杂十八烷 硅烷偶联剂G-407 双[γ-(三乙氧基硅)丙基]-四硫化物
英文名称：16-Dioxa-8,9,10,11-tetrathia-4,15-disilaoctadecane, 4,4',15,15-tretraethoxy-3
Coupling agent G-407
9,10,11-tetrathia-4,15-disilaoctadecane, 4,4,15,15-tetraethoxy-16-dioxa-8
bis[3-(triethoxysilyl)propyl]tetrasulfide
3,16-Dioxa-8,9,10,11-tetrathia-4,15-disilaoctadecane,4,4',15,15-tretraethoxy-
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条