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1)  Uncultured microorganisms
未培养微生物
1.
Therefore the microorganisms and especially uncultured microorganisms should be studied thoroughly, because they are important and necessary for researching microbiolog.
未培养微生物是指迄今在实验室的条件下无法纯培养的微生物。
2.
The extremophiles belong to a huge and potential superiority gene resource library and the research of uncultured microorganisms has been very important in the extremophiles research.
极端微生物是一个巨大的潜在的优势基因资源库,未培养微生物研究是极端微生物研究的一个重要组成部分。
3.
Cells of uncultured microorganisms from self-made compost were isolated by differential centrifugation, metagenomic DNA of which was extracted, end-blunted and ligated with cosmid vector of pWEB::TNC to construct a metagenomic library containing about 50, 000 clones.
通过差速离心从自制堆肥样品中分离未培养微生物细胞,提取其总DNA(metagenomic DNA),末端平头化后,用柯斯质粒pWEB::TNC为载体构建成了包含约5万个克隆的未培养微生物的宏基因组文库(metagenomic library),随机挑取其中的14个克隆提取质粒用BamHI酶切分析,结果显示所有的质粒都含有插入片段,最大为45kb,最小为25kb,平均大小为35kb,文库外源DNA总容量是1。
2)  uncultured microorganism
未培养微生物
1.
The total DNA of uncultured microorganisms from the enrichment cultures of the contents of buffalo rumens was isolated.
从水牛瘤胃内容物的添加滤纸为碳源的富集培养物中提取未培养微生物的总DNA,以柯斯质粒为载体构建了1个含约8000个克隆的宏基因组文库,对文库进行活性筛选获得1个既表达CMCase活性又表达4-MUCase酶活性的克隆。
2.
8×10~6 kb metagenomic DNA of uncultured microorganisms from the contents of buffalo rumens were constructed,and 118 independent clones expressingβ-glucosidase activity were isolated from the libraries.
本工作分6次构建了水牛瘤胃未培养微生物宏基因组文库,获得1。
3.
Based on the strategy to explore microbial resource from natural environment and the fact that the majority(about 99%) of microbes in diverse environments are unculturable till now by using traditional microbiological methods,scientists try hardrounding the bottleneck to set up novel techniques to culture and study those uncultured microorganisms.
在微生物筛选过程中,99%的未培养微生物是用传统的方法无法分离的,这极大地限制了微生物生态领域中的多样性和利用生物资源等方面的研究。
3)  Nonculturable microorganism
未培养微生物
4)  unculturable microorganism
未培养的微生物
5)  As-yet uncultured microorganisms
未被纯培养微生物
6)  Uncultured microbes
未获得培养微生物
补充资料:微生物培养法
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性质:在人为条件下使微生物发酵培养而达到繁殖的方法。根据各种微生物对养料、温度、空气、水分、酸碱度等条件的不同要求,可分别设计不同的培养方法,如高温培养法、通气培养法、厌气培养法等。

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