We constructed the recombinant transfer vector pTK-VP-IFNγ by inserting the goat IFN-γ gene into pTK-VP1 which contains the FMDV vp1 gene,The pTK-VP-IFNγ vector was purified and transfected by lipofection into bovine testicle cells that was infected with rAV41-LacZ expressing Escherichia coli β-Galactosidase (LacZ).

Cloning,phylogenetic evolution and expression of rabbit IFN-γ gene;

One pair of primers was designed according to duck interferon-gamma(IFN-γ)gene sequences published in GenBank,which was used to amplify the IFN-γ gene from total RNA extracted directly from Gushi duck splenocyte without induction of PHA by reverse transcription-polymerase chain reaction(RT-PCR).

From the total RNA,an IFN-γ gene of 435bp was amplified by RT-PCR.

After the total RNA was isolated from the lymphocytes, the porcine IFN-γgene was amplified by reverse transcription polymerase chain reaction (RT-PCR).

The result indicates that the cloned gene was acomplete PoIFN-γgene.

Construction of recombinant adenoviral vector co-expressing G gene of RV and IFN-a gene;