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1)  Luman-N-Term fusion protein
Luman-N-端融合蛋白
1.
To evaluate immunonicity of Luman-N-Term fusion protein,BALB/C mice were immunized intramuscularly with them and antigen specific antibodies and lymphocyte proliferative response of immunized mice were detected.
结果显示:细菌经IPTG诱导后在56 kD处,出现了特异性蛋白条带,与预计融合蛋白大小一致,融合蛋白经电洗脱纯化后免疫小鼠,间接ELISA显示抗体效价达到1∶4000,淋巴细胞增殖试验表明与空白组相比,Luman-N-端融合蛋白质量浓度为10、20、40和60μg/mL时,能显著刺激淋巴细胞的增殖(P<0。
2)  fusion protein
融合蛋白
1.
Molecular design and characterization of recombinant fusion protein of human IFNα_(2a)-THYα_1;
重组人干扰素α_(2a)和胸腺肽α_1融合蛋白的分子设计及其基因表达产物的鉴定
2.
Expression and purification of glycerol dehydratase β-subunit in E.coli as fusion protein;
甘油脱水酶β亚基融合蛋白在大肠杆菌中的表达和纯化
3.
Pro-apoptotic effect of recombinant anti-HER2 fusion protein ScFv/tBid gene on osteosarcoma E10 cells;
重组抗HER2融合蛋白基因ScFv/tBid对骨肉瘤E10细胞的促凋亡作用
3)  Fusion proteins
融合蛋白
1.
Separation and purification of recombinant fusion proteins of IFNα_(2b)-THYα_1;
重组人干扰素α_(2b)和胸腺肽α_1融合蛋白的分离纯化
2.
After induction by isopropyl β-D-1-thiogalactopyranoside(IPTG),the observed yield of crude fusion proteins was up to 260 mg/L.
将构建的含有胸腺肽α1融合蛋白的编码基因插入表达质粒pET28a(+)中,并在大肠杆菌BL21中进行表达,该菌经IPTG诱导融合蛋白高表达后,离心收集菌体,超声波破碎,包含体裂解,目标蛋白质的体外变性复性后,经DEAE-Sepharose Fast Flow阴离子交换层析纯化,每升菌液中可获得260 mg融合蛋白。
3.
Objective: To express the fusion proteins containing VP3 and Tat-PTD (protein transduction domain) proteins, and then purify and dialysis on this fusion proteins.
目的:原核表达VP3与Tat-PTD(protein transduction domain)的融合蛋白,并纯化此融合蛋白,观察Tat-PTD介导VP3进入胃癌细胞SGC7901的透膜效应及VP3在肿瘤细胞中的定位,检测融合蛋白诱导细胞凋亡情况。
4)  recombinant protein
融合蛋白
1.
Objective To explore the reasons for the low intracellular transduction efficiency of a previously constructed His-TAT-Flag recombinant protein and establish a more efficient transduction system.
目的探索本室构建的pET14b-His-TAT-Flag重组载体表达的融合蛋白在细胞中转导效率低的原因,建立高效的细胞内转导系统。
2.
The purified recombinant protein was analyzed by SDS-PAGE and Western blotting,the results showed that the CP fragment was highly expressed.
重组蛋白经过Ni+-NTA纯化后,SDS-PAGE和Western blotting分析结果表明,TRSV-CP基因在大肠杆菌中获得了高效表达,产生的融合蛋白分子质量约为34 ku,并具有免疫学活性。
3.
A recombinant protein with about 29kD was expressed stably in the form of insoluble inclusion bodies after IPTG induction.
PCR扩增拟南芥MnSODcDNA的保守区段,构建pET-SOD重组质粒,转化大肠杆菌JM109穴DE3雪,IPTG诱导融合蛋白高效表达;经检测表达产物占菌体总蛋白的69%,且以不溶的包涵体形式存在;表达产物变性后经Ni-NTASuperflow亲和柱分离纯化,得到相对分子质量约为29000的PAGE纯蛋白。
5)  fused protein
融合蛋白
1.
Expression of fused protein A-green fluorescent protein (PA-GFP);
蛋白A-绿色荧光蛋白融合蛋白的构建与表达
2.
AIM: To construct the prokaryotic recombinant plasmid to express HBV truncated C gene with preS1 gene and to acquire and purify the fused protein for antigenic analysis.
目的:构建乙型肝炎病毒(HBV)截短C基因和前S1基因重组原核表达质粒,获得融合蛋白的表达并进行抗原性分析。
3.
A method for the purification of expressed maltose binding protein fused protein by using potato starch instead of crosslinked amylose have been developed.
用马铃薯淀粉柱可以直接分离麦芽糖结合蛋白乳酸脱氢酶辅酶结合结构域融合蛋白,并得到满意的结果。
6)  chimeric protein
融合蛋白
1.
Objective To determine the free thiols in the chimeric protein PfCP-2.
目的 检测由毕氏酵母表达的恶性疟原虫融合蛋白(Plasmodium falciparum chimeric protein)PfCP-2。
2.
Objective To construct a chimeric protein of Plasmodium falciparum pre erythrocyte stage (named as PfCP 4).
目的 构建恶性疟原虫红前期融合蛋白 4(PfCP 4)。
3.
The results of activity assays showed that the chimeric protein could significantly promote the survival of spinal cord neurons and had a higher neurotrophic activity than ACTH(4 10) and GDNF respective.
通过PCR方法构建了促肾上腺皮质激素 4 10 (ACTH (4 10 ) )与胶质细胞源性神经营养因子 (GDNF)的融合基因 ,并将它重组克隆到表达载体 pET 2 8a (+ )中 ,构建表达质粒pET ACTH (4 10 ) GDNF ,转化大肠杆菌BL2 1(DE3) ,经IPTG诱导可高效表达ACTH (4 10 ) GDNF融合蛋白 。
补充资料:融合蛋白
分子式:
CAS号:

性质:两个不同的蛋白质连成一个大分子。可以通过化学方法连接,也可通过基因的融合来实现。在基因操作中,对一些分子数小的多肽基因常采用融合的方法与某一基因(如lac)相连,二者之间接上某一酶(如凝血酶)的切口,以增加在体内表达后产物的稳定性,也有的故意使两个分子串连融合以提高疗效,如IL-3与GM-CSF。也有与分泌性蛋白的信号肽基因组成融合基因,以使表达产物分泌到膜外或胞外。

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