1) Chromosome walking
染色体步行
1.
The PCR amplification techniques for chromosome walking;
用于染色体步行的PCR技术
2.
This paper reviews the existing chromosome walking PCR techniques,such as inverse PCR,panhandle PCR,ligation mediated(LM) PCR,thermal asymmetric interlaced(TAIL) PCR and single oligonucleotide nested(SON) PCR,and also puts forward a new chromosome walking method.
染色体步行是一种常用的克隆已知基因旁侧序列的技术。
2) genomic walking
染色体步行
1.
In the study,PS gene specific primers were designed,and the upstream sequence of PS gene was cloned by genomic walking based on ligation-mediated PCR method.
本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。
3) chromosome walking
染色体步查，染色体步移
4) Chromosome walking
染色体步查
1.
In an effort to clone a rice blast resistance gene, Pi*2(t), a BAC contig consisting of 22 BAC clones covering the whole Pi-2(t) region, was constructed usingmarker-based chromosome landing and chromosome walking.
应用BAC文库，采用基于分子标记的染色体着陆（marker-based chromosome landing）和染色体步查（chromosome walking）等手段，建立了包含有水稻抗稻瘟病基因Pi-2（t）的物理图谱，该物理图谱由22个BAC克隆组成，遗传跨度8cM，而物理距离为925kb，该物理图谱的构建不仅为进一步分离和克隆该基因打下了基础，同时也可为分子标记辅助选择育种选择抗稻瘟病新材料提供新的标记。
5) chromosome walking
染色体步移
1.
This paper introduced the basic principle of chromosome walking and taken actin gene of Pinus radiata as an example,conducted upstream and downstream chromosome walking based on the known EST sequence.
用染色体步移技术(chromosome walking)的基本原理以辐射松(Pinus radiata)肌动蛋白基因(actin)为例,利用获得的EST序列设计定向引物,向上游和下游进行了染色体序列的步移。
2.
Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region.
各种建立在PCR基础上染色体步移的方法能够根据已知的基因序列得到侧翼的基因序列。
3.
Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物PCR是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
6) Genome Walking
染色体步移
1.
This experiment used the method of Genome Walking(Chromosome Walki
本实验通过使用染色体步移法扩增得到了紫花苜蓿光敏色素A和光敏色素的5\'端序列,长度分别为1309bp和1752bp,补齐了之前实验所欠缺的5\'端编码序列。
2.
In order to explore the mechanism of porcine SP-A gene transcription regulation by studying the promoter region of porcine SP-A gene,the upstream sequence of Large White porcine SP-A gene was attained by Genome Walking PCR,sequencing and sequences alignment analysis.
为了探明猪SP-A基因的启动子及其转录调控机制,设计6条特异性引物,采用染色体步移、克隆测序以及序列比对分析,从大白猪基因组中扩增出一段长度为1 033 bp的猪SP-A基因的上游序列(DQ985806),其GC含量约为55%。
3.
A target fragment with a full length of 780 bp was amplified using genome walking.
根据已测得的该拮抗菌外泌抗菌肽氨基酸序列中的一段设计简并引物,利用染色体步移法得到全长序列780bp,经测序和BLAST比对分析表明,该基因属于CodY家族,与已鉴定的转录因子抑制剂CodY(ZP_01171531)的同源性最高;表现在氨基酸水平的相似性为77%,其序列全长已提交GenBank,登录号为FJ613128。
补充资料：染色体21-三体综合征
染色体21-三体综合征
病 名。即21-三体综合征，详见该条。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条