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1)  Akabane virus
赤羽病毒
1.
Eukaryotic expression of Akabane virus G1 gene;
赤羽病毒G1基因的真核表达及抗原性检测
2.
Using rabbit anti-Akabane virus recombinant nucleotide caspid protein serum as first antibidy,detecting virus antigen in inoculated BHK-21 cell and brain of baby mice by indirect Fluorescent Antibody test.
以兔抗赤羽病毒重组核蛋白血清作一抗,利用间接荧光抗体法在AKAV感染的BHK-21培养细胞和攻毒的乳鼠脑组织中特异性地检测到了AKAV抗原,为赤羽病的病原学诊断提供了简便的检测方法。
3.
The AGID test has been developed for the detection of antibody to Akabane virus in serum.
应用引自美国和日本的赤羽病毒和标准阳性血清 ,制备了琼脂免疫扩散试验 (AGID)抗原和高免阳性血清 ,建立了赤羽病AGID诊断方法。
2)  Akabane virus
赤羽病病毒
1.
Expression and Purification of Truncated Glycoprotein G1 Gene of Akabane virus and Development of an Indirect ELISA;
赤羽病病毒囊膜糖蛋白G1基因的截短表达、纯化及间接ELISA诊断方法的建立
2.
Development of an indirect ELISA for detection of Akabane virus infection using recombinant nucleocapsid protein;
赤羽病病毒核衣壳蛋白抗体间接ELISA检测方法的建立
3.
Expression,purification and identification of Akabane virus nucleocapsid protein;
赤羽病病毒核衣壳蛋白基因的表达纯化及活性鉴定
3)  akabane disease
赤羽病
4)  Scab pathotoxin
赤霉病菌毒素
5)  tetronerythrin
羽赤素
6)  sweet potato feathery mottle virus
甘薯羽状斑驳病毒
1.
The cDNA of SPFMV coat protein gene was synthesized by RT-PCRwith artifically synthesized two specific primers and the RNA of sweet potato feathery mottle virus Chinese isolate as templete.
根据已报道的甘薯羽状斑驳病毒(Sweetpotatofeatherymottlevirus,SPFMV)外壳蛋白基因序列,设计合成了一对特异性引物,以我们从国内甘薯品种徐薯-18上分离到的SPFMV的RNA为模板,经过反转录合成cDNA第一条链,经PCR扩增、限制性酶切后克隆于pUC19的Hind 和SacI位点,转化大肠杆菌JM109,经限制性酶切分析、PCR鉴定以及序列分析证实获得了SPFMV中国分离株外壳蛋白基因的全长克隆。
2.
According to published nucleotide sequence, coat protein gene of sweet potato feathery mottle virus (SPFMV) isolated from China was synthesized by reverse transcripation-polymerase chain reaction (RT- PCR ) and constructed into expression vector pET30a (+) for overexpression in prokaryotic cells.
根据已报道的甘薯羽状斑驳病毒(SPFMV)外壳蛋白(CP)基因的核苷酸序列合成引物,利用RT-PCR的方法获得CP基因后,再将其克隆到原核表达载体pET30a(+)中。
3.
Sweet potato feathery mottle virus (SPEMV) was purified from infected I.
从感染有甘薯羽状斑驳病毒的牵牛(Ⅰ。
补充资料:赤羽
1.赤色羽毛。 2.指羽毛赤色的禽鸟。 3.羽箭名。铁镞。 4.赤色旗帜。
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