1) goat oocytes
山羊卵母细胞
1.
The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate,pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.
结果表明:6-DMAP能显著提高山羊卵母细胞的激活率,乙醇+6-DMAP和离子霉素+6-DMAP处理卵母细胞激活率分别为75。
2) goat oocyte
山羊卵母细胞
1.
Effect of cytochalasin-B and sucrose and mature time on goat oocytes enucleation;
CCB、蔗糖及成熟时间对山羊卵母细胞去核效果的影响
2.
In present study,comparisons were carried out to develop and improve in vitro mauration(IVM) systems of goat oocytes.
为了通过比较培养筛选出能改进和提高山羊卵母细胞体外成熟效率的培养体系 ,培养的山羊卵母细胞以 TCM-199为基础培养液 ,添加 :(1) 10 %血清 (胎牛血清 (FBS)或发情山羊血清 (EGS) ) +2 0 m g/ L促黄体素 (L H) +10 mg/ L促卵泡素 (FSH) +1m g/ L 雌二醇 (E2 ) ;(2 ) 10 % EGS+促性腺素 (L H∶ FSH=5 mg/ L∶ 0 。
3) Sheep oocytes
绵羊卵母细胞
1.
The objective of present study were to compare parthenogenic development of sheep oocytes activated by different sequential treatments with either 7% ethanol or 5mM ionomycin, and either 2mM 6-dimethylaminopurine (6-DMAP) or 6-DMAP+ 5μg/mL cytochalasin B(CB).
本研究利用常规化学激活剂离子酶素(Ionomycin)和乙醇分别与6-二甲基氨基嘌呤(6-Dimethylaminoputine,6-DMAP)和细胞松弛素B(CB)的不同组合对绵羊卵母细胞的激活后体外发育效果进行了比较。
4) Ovine oocyte
绵羊卵母细胞
1.
Effect of the inhibitor of cdk and specific phosphodiesterase isoenzyme inhibitors on ovine oocytes meiotic maturation in vitro;
磷酸二酯酶亚型抑制剂与细胞周期蛋白抑制剂对绵羊卵母细胞体外成熟的影响
2.
The effects of TGFα and EGF on nuclear and cytoplasm maturation of ovine oocytes cultured in basical medium + BSA, serum, BSA+EGF and BSA+TGFα respectively were investigated by immunofluores-cence and orcein staining.
采用地衣红染色和免疫荧光的方法,观察了培养在基础培养液加BSA、血清、BSA+EGF和BSA+TGFα四组成熟培养液中绵羊卵母细胞的核成熟状态和α-微管蛋白分布,以及成熟培养后皮质颗粒(CG)的分布情况。
3.
This study was designed to examine the ultrastructural changes of ovine oocytes classified according to follicular size and layers of cumulus cells before and after in vitro culture.
实验利用透射电镜研究了根据卵泡大小和卵丘细胞层多少分类后各类绵羊卵母细胞在体外成熟培养前后超微结构的变化 ,结果表明 :小卵泡 (直径小于 1mm)卵经过体外培养其线粒体、皮层颗粒等细胞器均未达到成熟卵母细胞中这两种超微结构的分布状态 ;大、中两类卵泡(直径大于 3mm和 1 3mm)卵经过体外培养细胞质成熟较好 ,但其线粒体的分布与体内成熟卵稍有差异 ,说明当前的培养系统并不能支持卵胞质充分成熟 。
6) oocyte
[英]['əuəsait] [美]['oə,saɪt]
卵母细胞
1.
Effects of sucrose of different concentrations on GV and MⅡ vitrificated oocyte spindle;
蔗糖浓度对GV和MⅡ期冻融卵母细胞纺锤体结构的影响
2.
Expression of trophinin in human oocytes and preimplantation embryos;
Trophinin在人卵母细胞及着床前胚胎细胞上的表达与意义
3.
Epidermal growth factor improves in vitro maturation of immature mouse oocytes;
表皮生长因子促进小鼠未成熟卵母细胞体外成熟的实验研究
补充资料：不成熟卵母细胞体外成熟
不成熟卵母细胞体外成熟
是将表皮生长因子和促性腺激素联合用于不成熟卵母细胞进行体外培养，提高受精率及提高不成熟卵母细胞的体外成熟率。它是一项助孕技术，可以减少超排卵带来的经济负担和卵巢过度刺激的危险。解决卵巢本身缺陷诱发排卵无效病人的生育问题，以及为“卵子指赠”工作提供卵母细胞来源，但体外成熟的卵母细胞的胚胎较体内成熟卵母细胞要差，因此必须了解影响卵母细胞体外成熟能力因素和改善培养环境。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条