Since roundup ready soybean introduction in 1996, it has been adopted by growers at a rapid rate in the world.

Objective:To establish qualitative and quantitative PCR methods to detect the Roundup Ready soybean in foods and to explore the qualitative method to detect the genetically modified components in soybean oil through improving the DNA extraction method.

By using transmission electron microscope(TEM) and scanning electron microscope(SEM),we studied ultrastructural changes in photosynthesis organ-leaves of glyphosate-resistant soybean and soybean Dongnong42,in response to glyphosate.

Different dosage of Roundup41% aqua and Glyphosate10% aqua were sprayed during flowering and podding periods,and chlorophyll content,stomatal conductance,transpiration rate and photosynthetic rate of glyphosate-resistant soybean(RR2) were monitored.

【Method】 In the field,the surviving competition ability(including germination rate,plant height,leaf number),reproducing ability(the number of flowers,legumes,and seeds per plant),volunteer possibility,seed shattering and persisting possibility of transgenic glyphosate-resistant soybean 40-3-2,recipient variety and conventional soybean varieties were comparatively evaluated.

The genotypes 7039 and 7040 were used to select regenerated plants which showed glyphosate resistance and the other two genotypes were used for haloxyfop resistance selection.

In this study, Glyphosate resistance gene has been carried out genetic analysis and marked, losated by the use of SSR molecular markers ,with the Northeast Agricultural University glyphosate resistant strains 89-09 and 89-05-3, and susceptible strains 89-13 and 89-21 for the test materials, through the F2 groups of the two sets of hybrid combinations 89-05-3/89-13 and 89-09 / 89-21.

Metabolism and deposition of exogenous DNA from transgenic glyphosate-tolerant soybean meal in grower pigs were studied in the experiment.