1) two-dimensional electrophoresis
双向电泳技术
1.
The effects of different protein extraction methods(phenol extraction method and TCA-acetone precipitation method),and different lysis buffer on two-dimensional electrophoresis(2-DE) gels were compared,the loading quantity of sample and the gel concentrations were also optimized for the analysis of 2-DE system on the monokaryotic stage anther of wheat.
优化后的双向电泳技术体系,适合于小麦花药全蛋白质的双向电泳分析。
2.
Recently,two-dimensional electrophoresis with immobilized pH gradients combined with protein identification by mass spectrometry and the analysis of databases is currently the main system for proteomic research.
对蛋白质组学研究中的三大核心技术——双向电泳、生物质谱、生物信息学技术的进展及其应用进行了概述,重点介绍了双向电泳技术和生物质谱技术。
2) two-dimensional electrophoresis and mass spectrometry
双向电泳-质谱技术
4) 2-DE
双向电泳
1.
Analysis of HSP70 protein polymorphism with 2-DE and Western-blot;
人淋巴细胞HSP70蛋白多态性双向电泳-Western blot分析
2.
The establishment of the two dimensional gel electrophoresis (2-DE) map of hypertrophic scar epidermal cells and the display of the differentially expressed proteins;
增生性瘢痕表皮细胞双向电泳图谱的建立及差异蛋白展示
3.
Preliminary establishment of two-dimensional gel electrophoresis(2-DE) for muscle tissue of bloody clam Tegillarca granosa;
泥蚶肌肉组织蛋白质双向电泳体系的建立
5) two-dimensional electrophoresis
双向电泳
1.
Comparative study of three thymic protein refining methods in two-dimensional electrophoresis;
3种方法提取胸腺组织蛋白双向电泳纯化结果比较
2.
Mechanism of hepatocyte transformation by HCV NS3 using two-dimensional electrophoresis and mass spectrometry;
双向电泳和质谱技术分析HCV NS3转化肝细胞的蛋白质组
3.
Analysis of two-dimensional electrophoresis maps of congenital total cataractous and normal lens proteins;
先天性全白内障晶状体和透明晶状体蛋白双向电泳差异比较
6) two dimensional electrophoresis
双向电泳
1.
Proteome of human CD4~+CD25~+T cells vs CD4~+CD25~-T cells: Comparative analysis by two dimensional electrophoresis;
人CD4~+CD25~+T细胞和CD4~+CD25~-T细胞蛋白质组的双向电泳分析
2.
The application of two dimensional electrophoresis in the separation of proteins in Bombyx mori;
双向电泳法在家蚕蛋白质分离中的应用
3.
Here,we present a method for the proteomic analysis of CNB sample based on the two dimensional electrophoresis.
本文建立和优化了适合针刺活检样品的基于双向电泳的蛋白质组学分析平台:通过直接抽提获得针刺活检组织的蛋白质样品;用24cm固定梯度干胶条(pH3-10NL)等电聚焦及12。
补充资料：双向电泳
分子式：
CAS号：
性质：以等电聚焦作为第一向，将蛋白质按等电点不同分开，然后将已按等电电点分开的蛋白质进行SDS-聚丙烯酰胺凝胶电泳作为第二向，让等电点相同或相近的蛋白质再按分子量大小分开。双向电泳使分辨率大大提高，若结合同位素标记等技术，分辨率将会更高，可将多蛋白组分中等电点或分子量相同或相近的蛋白质分开。
CAS号：
性质：以等电聚焦作为第一向，将蛋白质按等电点不同分开，然后将已按等电电点分开的蛋白质进行SDS-聚丙烯酰胺凝胶电泳作为第二向，让等电点相同或相近的蛋白质再按分子量大小分开。双向电泳使分辨率大大提高，若结合同位素标记等技术，分辨率将会更高，可将多蛋白组分中等电点或分子量相同或相近的蛋白质分开。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条