A novel population consisted of 86 single segment substitution lines (SSSLs) were developed from advanced backcrosses between the recipient,Huajingxian 74 and six donors by microsatellite marker-assisted selection (MAS).

The quantitative trait loci (QTLs) for 21 traits of agronomy importance on ten substituted chromosomal segments were identified using single segment substitution lines (SSSLs) in rice,which were developed by the use of Taichung 65 as a recipient and Zhai-ye-qing and Dee-geo-woo-gen as donors respectively.

【Objective】 Single segment substitution lines(SSSLs) each with only single chromosome segment from a donor under the same genetic background as the recipient were developed in rice by advanced backcrossing and molecular marker-assisted selection.

QTLs can be divided into several simple Mendelian factors using single segment substitution lines (SSSLs) with the characterizations of single substituted segment, purifying of genetic background, genetic stability and so on.

The single segment substitution lines(SSSLs) were perfect materials for analysis of gene especially for QTL.

Twenty-nine single segment substitution lines (SSSLs) with the recipient, Taichung 65 and the donor, Dee-geo-woo-gen or Zhaiyeqing had been developed through simple sequence repeat (SSR) marker-aided selection.