The major response surface and some sub-response surfaces are adopted in the method.

Application of response surface methodology in the optimization of solid-phase extraction condition for the determination of trans-resveratrol in rat urine;

Based on the results of single factor experiment,a three-level central composite design was employed combining with response surface methodology to optimize the ferment conditions for yield of lactic acid.

Better transesterification conditions of rapeseed oil were presented by single factor and response surface methodology analysis.

The Response Surface Methodology (RSM) was employed to establish the model of Instantaneous High Pressure inactivation Bacillus subtilis.

Response surface methodology(RSM) based on a five-level five-factor central composite rotatable design of experiments was used to optimize the optimal levels of five important factors,namely temperature(X1),pH value(X2),concentration of methamidophos(X3),the medium volume(X4),the quantity of inoculum(X5).

Based on the results,Response Surface Methodology(RSM) was employed and a quadratic polynomial equation for the hardness and water holding capacity of tofu was built in the present work.

Based on single factor test,response surface methodology(RSM) was used to study effects of extraction time,ethanol concentration and solid-liquid ratio on extraction rate of total flavonoids.