2) lethality coefficient
致死系数
4) Balanced lethal system
平衡致死系统
1.
pylori with balanced lethal system MethodsThe gene of urease B subunit of H pylori was amplified and recombined with a vector(asd +) The recombinant was transformated into attenuated salmonella typhimurium The expression of urease B subunit was analysed by SDS PAGE and western blot, also the stability of vaccine strains was investigated by generation culture in vitro ResultsAn a.
目的采用平衡致死系统构建表达幽门螺杆菌尿素酶 B亚单位的减毒沙门氏菌工程菌株。
2.
In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd? attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS.
将生长抑素(SS)与乙肝表面抗原(S)融合基因插入平衡致死系统原核表达质粒pYA3493中,转化至缺失asd基因的减毒猪霍乱沙门氏菌C500,经酶切、测序筛选得到非抗性的目的克隆,命名为pYA-SS。
5) balanced-lethal system
平衡致死系统
1.
The construction and application of chromsome-plsmid balanced-lethal system;
染色体-质粒平衡致死系统的构建及应用
6) asd-balanced lethal vector system
asd平衡致死系统
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