EXPRESSION OF HUMAN MUTANT CD59 IN CHO CELL;

Objective To study over expression of VMAT_(2) in CHO cells resisted toxicity of MPP~(+) and rotenone.

The recombinant vector PCI-neo-PoIFN-γ wased transfer into Chinese hamster ovary cells (CHO) using lipofectamine the strain was screened,and screening training in the presence of G418.

Establishment of stable Chinese hamster ovary cells expressing of high-lever hHGF;

Functional study of human TSH receptor transfected into Chinese hamster ovary cells;

Methods The viable region of light chain(VL) and viable region of heavy chain(VH) genes of anti-DR5 antibody were amplified and cloned into the light-and heavy-chain expression vectors respectively,then the recombinant plasmids were co-transfected into dihydrofolate reductase-Chinese hamster ovary cell(CHO-dhfr-) for expression.

In order to further study the method for screening GR antagonists, GR cDNA was transiently transfected into CHO cells, and then fluo-3 was loaded into.

Objective To compare the protective effects of Daidzein and Genistein against H2O2 induced oxidative damage in CHO cell line of healthy subjects and to investigate the antioxidant activity of isoflavones.

The standard and mutated human PrP genes were separately inserted into an eukaryotic expression vector and transfected into CHO cell, respectively, generating CHOs strain containing the standard PrP gene and CHOm strain containing the mutated PrP gene.