1) oligonucleotide library
寡核苷酸文库
1.
coli M15 to generate soluble GST-Mip1 fusion protein,and the GST-Mip1 was purified by glutathione affinity chromatography and immobilized on Sepharose beads to capture putative target DNA binding site from the oligonucleotide library.
通过固定化的GST-Mip1,从寡核苷酸文库中俘获Mip1的结合的寡核苷酸片段;将所得寡核苷酸片段插入T-载体,通过测序、序列比对得到DNA结合位点。
2) random oligonucleotide library
随机寡核苷酸文库
1.
To identify antisense accessible sites of survivin,the 20 mer random oligonucleotide library was synthesized and hybridized with in vitro transcripted total survivin cRNA,then digested by RNase H.
合成 2 0mer随机寡核苷酸文库 ,与体外转录出的全长survivincRNA杂交 ,RNaseH酶切割后 ,经引物延伸、放射自显影 ,共筛选出 13个针对survivin基因的反义结合位点 (antisenseaccessiblesites ,AAS) 。
4) Random oligo-nucleotide bank
随机寡核苷酸库
5) Oligonucleotides
寡核苷酸
1.
Spectroscopic Study on Sequence Specific Binding of Thionine with Oligonucleotides;
硫堇与寡核苷酸结合序列选择性的光谱研究
2.
Effect of Column Temperature and Flow Rate on Retention and Separation of Oligonucleotides by Reversed Phase Ion Pair Chromatography;
柱温和流速对反相离子对色谱法分析寡核苷酸的影响
3.
Biological effect of c-myc antisense oligonucleotides on MKN-45 cells;
c-myc反义寡核苷酸对胃癌MKN-45细胞株生物学影响的研究
6) oligodeoxynucleotides
寡核苷酸
1.
Effects of NF-κB “decoy” oligodeoxynucleotides on inducible nitric oxide synthase expression in LPS-induced mouse macrophages;
NF-κB“诱骗”寡核苷酸对小鼠巨噬细胞iNOS表达的影响
2.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of oligodeoxynucleotides consisting of isonucleosides with sinapinic acid as matrix was reported.
以芥子酸（sinapinicacid）为基质，对异核苷杂寡核苷酸（3－（R）－IsodT）13·dT和（3－（S）－IsodT）13·dT进行了基质辅助激光解吸电离飞行时间质谱测定，准确地测得分子量，合成的寡核苷酸得以鉴定。
3.
Objective To study the effect of NF-κB decoy oligodeoxynucleotides on IL-8 expression in LPS-induced SW480 cells.
目的探讨核转录因子κB(NF-κB)"诱骗(decoy)"寡核苷酸(ODNs)对结肠癌SW480细胞IL-8表达的影响。
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性质： 见低聚核苷酸
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性质： 见低聚核苷酸
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