1) antibody humanization
抗体人源化
1.
Subsequently, its structure is modified for humanization and a quick method is thus proposed for antibody humanization.
通过计算机辅助分子设计的手段 ,综合序列分析和结构分析的结果对抗体的结构完成了计算机模拟 ,并对其进行了人源化的模建和改造 ,从而建立了一套快速可行的抗体人源化设计方案 。
2) Humanized antibody
人源化抗体
1.
The expression of humanized antibody containing mutated Fc region (hu12F6m) was confirmed by ELISA and RT-PCR.
为了降低抗CD3人源化抗体hu12F6对T细胞的激活作用以克服首剂效应 ,构建了恒定区特定位点突变的hu12F6重链表达载体 ,将其与hu12F6的轻链表达载体共转染CHO细胞 。
2.
Binding of the humanized antibody to the EGFR was assessed by Surface Plasmon Renainance with Biacore3000,and the biological activity of the humanized antibody was determined by tumor cell invasion test.
结果:成功构建3种不同突变体表达载体C2、C3和C5,纯化的抗体在还原SDS-PAGE中表现为相对分子质量约为25×103和50×103两条带;免疫印迹分析表明,该人源化抗体可与羊抗人IgG特异性结合。
3) humanized scFv
人源化单链抗体
5) humanized anti-HER2 monoclonal antibody
抗HER2人源化单克隆抗体
1.
Objective: To study whether the humanized anti-HER2 monoclonal antibody could be expressed in Pichia pastoris, and to investigate the structure and the activity of the expressed products.
方法:合成抗HER2人源化单克隆抗体的全基因,构建轻重链双表达盒表达载体,转入毕赤酵母中;通过表型筛选和诱导表达实验得到抗体表达工程菌,对表达产物进行分离纯化和活性测定。
6) Human antibody
人源抗体
1.
Ribosome display is a rapid means of generating fully human antibody fragments in vitro.
核糖体展示技术为体外筛选制备全人源抗体提供了一种简便、有效的技术平台。
补充资料：公理化方法(见公理化和形式化)
公理化方法(见公理化和形式化)
axiomatical method
gongllbuafangfa公理化方法化和形式化。(axiomatieal method)见公理
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条