1) Subtractive hybridization
减法杂交
1.
An improved method was utilized for constructing hybrid arrested λ phage cDNA library by subtractive hybridization, in which magnetic particles were applied to separate monomers from hybrids, and candidate sscDNAs were enriched by subtracting two control mRNAs from one treated sscDNA.
介绍了一种改进的利用减法杂交构建λ噬菌体ｃＤＮＡ文库的方法，该方法利用处理的ｓｃＤＮＡ减去两种对照的ｍＲＮＡ富集目的ｓｓｃＤＮＡ并用磁珠法分离杂交单体，结果表明其增加了杂交单体的产量、简化了实验步骤，而且更有利于富集所需基因，是构建ｃＤＮＡ文库的一个有效改进。
2) Suppression subtractive hybridization
抑制消减杂交法
1.
Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K.
方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。
3) subtractive cDNA probes
减法杂交探针
4) improved subtractive hybridization
改良消减杂交法
1.
In order to study the mechanism of hyperplasy and invasiveness of fibroblast\|like synovial cells(FLS)of rheumatoid arthritis(RA)patients,an improved subtractive hybridization was used to screen for the genes which expression was increased in RA FLS compared with FLS of osteoarthritis (OA) patients.
为了研究类风湿性关节炎 (rheumatoid arthritis,RA)滑膜细胞 (fibroblast- like synovialcells,FLS)过度增殖和破坏软骨的分子机理 ,利用改良消减杂交法以骨性关节炎 (osteoarthritis,OA)病人滑膜细胞为对照 ,筛选 RA滑膜细胞中的高表达基因 。
5) Subtractive hybridization
消减杂交
1.
Cloning of Novel Genes Related Apoptosis by Improved, PCR-based Subtractive Hybridization;
应用基于PCR的改良消减杂交技术克隆凋亡相关基因
2.
BACKGROUND & OBJECTIVE: Subtractive hybridization technology is a common method to screen and clone differentially expressed genes.
背景与目的:消减杂交技术是一种寻找和克隆差异基因的方法。
3.
Methods In our study, SMART reverse transcription method, long-distance PCR, streptavidin magnetic bead-mediated subtraction and spin column chromatography were adopted on the basis of classical subtractive hybridization.
目的 运用改良消减杂交技术构建大鼠脊髓损伤修复过程中出现的差异表达基因文库 ,为寻找在脊髓损伤后的修复过程中起关键作用的分子提供帮助。
6) Subtractive hybridization
差减杂交
1.
Here we use the method of subtractive hybridization to i.
方法：本文的研究是基于心肌肥厚过程中各种基因在表达上所发生的变化，选用腹主动脉绑扎的左心室肥厚大鼠为模型，运用差减杂交法，旨在分离心肌肥厚过程中的相关基因。
2.
In the library,588 novel expressed sequence tags(ESTs) were obtained through suppression subtractive hybridization(SSH),and most of the identified ESTs were reported for the first time in amphioxus.
应用抑制性差减杂交(SSH)技术,通过对差减文库克隆序列的测定,共获得588个表达序列标签(EST)。
补充资料：测定货币需要量简单加减法
测定货币需要量简单加减法
【测定货币需要.简单加减法】简单地运用实际供给的现金量与应该供给的现金量的比较结果来测算货币需求量的方法。中国工商银行总行曾用此法测算了1984年的货币需求量，其计算公式为: 多供给的现金量=实际供给的现金量一应该供给的现金量 其中，应该供给的现金量包括:商品流通扩大需要增加的现金量、城乡个体专业户增加需要扩大的现金量以及各单位需扩大的库存现金等。 该方法简单明了，易于统计。但是在现金需求量与货币需求量的对应关系中，忽视了现金与存款的转化关系;此外，应该供给的现金量受到多种因素制约，难以准确计量。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条