1) Herpes Simplex virus-thymidine kinase
单纯疱疹胸苷激酶
1.
Purpose:To extract and identify of pDR2-TK, an EBV-based expression vector, and to transfer the vector into human prostatic cancer cells by using liposome-mediated gene delivery technique, Herpes Simplex virus-thymidine kinase (HSV-TK) expression in the cancer cells were evaluated as well.
目的 :提取并鉴定已构建的EB病毒表达载体pDR2 TK ,利用脂质体介导的基因转染技术将 pDR2 TK转染人前列腺癌细胞并对单纯疱疹胸苷激酶 (HSV TK)表达状况进行检测。
2) HSV-tk gene
单纯疱疹病毒胸苷激酶
1.
Methods:By using Adeasy system, recombinant adenoviral plasmid containing KDR promoter-controlled HSV-tk gene (AdKDR-HSV-tk) was constructed.
HUVEC+HUVEC／tk混合细胞的存活率,随HUVEC／tk细胞比例的增加而降低,说明腺病毒介导的KDR启动子-单纯疱疹病毒胸苷激酶／丙氧鸟苷系统不但能杀伤tk+细胞,也能杀伤未导入tk的细胞,存在明显的旁观者效应。
2.
Methods: HSV-TK gene was transfected into PC-3m cells through retrovirus vector, reverse transcription-polymerase chain reaction(RT-PCR) was used to demonstrate the successful transfection and the transcription of HSV-TK gene.
目的:探讨单纯疱疹病毒胸苷激酶(herpessimplexvirus-thymidinekinase,HSV-TK)自杀基因体系联合重组干扰素α-2a(rIFNα-2a)对前列腺癌PC-3m细胞的体外杀伤作用。
3) hsv-tk
单纯疱疹病毒胸苷激酶
1.
Aim:To construct a recombinant eukaryotic expression vector containing α-fetoprotein(AFP) promoter-mediated HSV-TK suicide gene,and analyze its specific expression on AFP-positive hepatoma cells.
目的:构建甲胎蛋白(AFP)启动子介导的单纯疱疹病毒胸苷激酶(HSV-TK)重组真核表达载体,并检测其在AFP阳性肝癌细胞株的特异性表达。
2.
To improve the effectiveness of radiotherapy and chemotherapy to these tumours, a eukaryotic expression vector expressing herpes simplex virus-thymidine kinase (HSV-TK) regulated by hypoxia responsive element (HRE) under hypoxia was constructed.
本研究拟构建一种由缺氧反应元件(hypoxia responsive element，HRE)调控的单纯疱疹病毒胸苷激酶(herpes simplex virus-thymidine kinase，HSV-TK)基因的真核表达载体，观察HRE能否增强HSV-TK／丙氧鸟苷(gancyclovir，GCV)系统对乏氧环境中的人Ewing肉瘤细胞系(SK-ES-1细胞)的杀伤作用，为弥补放、化疗的不足探索有效的治疗方法。
4) Herpes simplex virus thymidine kinase
单纯疱疹病毒胸苷激酶
1.
Aim To observe in vitro cytotoxicities of GCV and CTL to rat mammary cancer cells transfected with herpes simplex virus thymidine kinase(HSV TK) and costimulatory molecule(B7) genes via retrovirus vector.
目的 观察羟甲基无环鸟苷 (GCV)、CTL对逆转录病毒介导的转染单纯疱疹病毒胸苷激酶 (HSV TK)和共刺激分子 (B7)双基因的大鼠乳腺癌细胞的体外杀伤作用。
2.
Objective: To investigate the role of herpes simplex virus thymidine kinase (HSV-tk) gene expression driven by vascular endothelial growth factor (VEGF) promoter followed by ganciclovir (GCV) administration in killing hepatocellular carcinoma(HCC) cells in vitro.
目的 :构建携带受血管内皮生长因子 (VEGF)启动子调控的单纯疱疹病毒胸苷激酶基因 (HSV- tk)的重组腺病毒载体 ,并探讨该重组腺病毒对体外培养的肝癌细胞的特异性杀伤活性。
3.
Under the current circumstance of cancer gene therapy, herpes simplex virus thymidine kinase (HSV-tk) suicide gene therapy is regarded as a promising strategy because it is independent of the genetic background of carcinogenesis, which is not clear yet, and.
当前，肿瘤基因治疗所面临的一个主要问题是基因载体的低效率，而单纯疱疹病毒胸苷激酶(HSV-tk)基因治疗中存在的旁观者效应则至少在一定程度上使该问题得以解决。
5) HSV-TK/GCV
单纯疱疹病毒胸苷激酶/丙氧鸟苷
1.
The application of HSV-TK/GCV gene therapy on bladder cancer;
单纯疱疹病毒胸苷激酶/丙氧鸟苷系统对膀胱癌体内杀伤作用的研究
6) Herpes Simplex virus thymidine kinase/ganciclovir
单纯疱疹病毒胸苷激酶/羟基无环鸟苷
补充资料：脱氧胸苷激酶
分子式：
CAS号：
性质：又称胸苷激酶。催化脱氧胸苷磷酸化形成胸苷一磷酸(dTMP)：该酶还催化脱氧尿苷磷酸化形成脱氧尿苷一磷酸(dUMP)。正常肝组织内该酶活性很低，在迅速生长的组织如再生肝内酶活性升高，肝恶性肿瘤组织内酶活性明显升高。
CAS号：
性质：又称胸苷激酶。催化脱氧胸苷磷酸化形成胸苷一磷酸(dTMP)：该酶还催化脱氧尿苷磷酸化形成脱氧尿苷一磷酸(dUMP)。正常肝组织内该酶活性很低，在迅速生长的组织如再生肝内酶活性升高，肝恶性肿瘤组织内酶活性明显升高。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条