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1)  Microwave ELISA
微波酶联免疫吸附试验
1.
Methods Microwave ELISA and routine ELISA were used to detect parallelly specific antibody in the sera of 262 patients with schistosomiasis japonica, 92 healthy individuals, 15 patients with clonorchiasis sinensis and 15 patients with paragonimiasis westermani.
目的 评价微波酶联免疫吸附试验 (微波 EL ISA)诊断日本血吸虫病的效果。
2)  ELISA [英][i'laizə]  [美][ɪ'laɪzə]
酶联免疫吸附试验
1.
Application of ELISA for microcystins detection;
酶联免疫吸附试验在微囊藻毒素检测中的应用进展
2.
Effection of Sample Factors to ELISA Detection;
标本因素对酶联免疫吸附试验测定的影响
3.
Common Factors of Influencing HBsAg and Countermeasure in ELISA;
酶联免疫吸附试验影响HBsAg常见因素与对策
3)  enzyme linked immunosorbent assay
酶联免疫吸附试验
1.
Evaluation of enzyme linked immunosorbent assay for the detection of West Nile virus antibodies;
西尼罗病毒抗体酶联免疫吸附试验检测方法的评价
2.
A double sandwich enzyme linked immunosorbent assay utilizing linear sweep polarographic detection (ELISA-LSP) for the detection and type identification of herpes simplex virus (HSV) antigen was established.
将酶联免疫吸附试验(ELISA)与单扫描示波极谱法(LSP)检测相联接,用自制的小型“DME-Pt-Ag/AgCl”三电极系统,与商品酶联板配套,直接检测酶促反应电活性产物,建立了ELISA-LSP法检测单纯疱疹病毒(HerpesSimplexVirus,HSV)抗原并进行分型的方法。
3.
Objective To explore the clinical value and relationship between time-resolved fluoroimmunoassay(TRFIA) to detect HBV M in quantity and enzyme linked immunosorbent assay(ELISA) to detect HBV M in quality.
目的探讨时间分辨荧光免疫测定法(TRFIA)定量检测乙型肝炎血清学标志物(HBVM)与酶联免疫吸附试验(ELISA)定性检测HBVM的关系及其临床意义。
4)  Enzyme-linked immunosorbent assay
酶联免疫吸附试验
1.
Comparison of enzyme-linked immunosorbent assay and direct fluorescent antibody for the detection of Chlamydia trachomatis
酶联免疫吸附试验和直接免疫荧光检测沙眼衣原体的方法学比较
2.
[Methods] PG Ⅰ and Ⅱ were measured with the two-site "sandwich" technique of enzyme-linked immunosorbent assay(ELISA).
目的确定胃蛋白酶原PGⅠ、PGⅡ酶联免疫吸附试验(ELISA)试剂盒的分析灵敏度及其对胃癌的诊断价值。
3.
METHODS: Enzyme-linked immunosorbent assay was used to measure the levels of serum HBV-LP, HBV preS1, HBV preS2 and HBV markers, and fluorescence quantitative-polymerase chain reaction (FQ-PCR) was used to detect HBV DNA in 162 patients with hepatitis B as well as 47 normal controls.
方法:对162例HBV感染者YL47A正常对照血清采用酶联免疫吸附试验检测HBV-LP,HBV 前S1抗原,HBV前S2抗原及乙型肝炎血清标志物:FQ-PCR定量检测HBV DNA。
5)  ELISA [英][i'laizə]  [美][ɪ'laɪzə]
间接酶联免疫吸附试验
1.
The positive clones were picked out and identified by ELISA and WB.
方法以中国莱姆病螺旋体伽氏疏螺旋体(Borreliagarinii)的代表菌株PD91的全菌蛋白为抗原,免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞SP2/0融合,用间接酶联免疫吸附试验(ELISA)和蛋白免疫印迹方法(WB)筛选,并经过2次或3次克隆,以获得单克隆抗体。
2.
Methods Serum IgM antibodies of influenza virus A and B(FluA and FluB),respiratory syncytial virus(RSV),adenovirus(ADV)and parainfluenza virus(PIV)were detected by indirect enzyme linked immunosorbent assay(ELISA).
方法采用间接酶联免疫吸附试验(ELISA)方法检测血清流感病毒A(FluA)、流感病毒B(FluB)、呼吸道合胞病毒(RSV)、腺病毒(ADV)和副流感病毒(PIV)的IgM抗体。
3.
leprae and their trends in sera from NC (192 cases) and CLP (666 cases, including 8 cases relapsed) in ELISAs using ND, Ms, LAM B and α 2 as antigens were mearsured.
方法 : 用间接酶联免疫吸附试验(ELISA)检测正常人 (192例 )和愈后原麻风病人 (6 6 6例 ,含 8例复发者 )血中抗体水平及其态势 ,计算各抗原检测结果间的个体符合率并加以比较。
6)  Enzyme-linked immunosorbent assay(ELISA)
酶联免疫吸附试验
1.
TTV-DNA and anti-TTV-IgG of the sera of the patients with hepatic diseases(132 cases) and the blood donors(120 cases) were detected simultaneously by polymerase chain reaction(PCR) and enzyme-linked immunosorbent assay(ELISA).
采用聚合酶链反应 (PCR)和酶联免疫吸附试验 (EL ISA) ,分别检测 132例各型肝病患者和 12 0例献血员血清 TTV- DNA和抗 TTV- Ig G。
补充资料:酶联免疫吸附试验


酶联免疫吸附试验


酶联免疫吸附试验是将可溶性抗原或抗体吸附(包被)于固相载体(目前常用微孔反应板)上,加入待检样品,所测未知抗体或抗原与包被的抗原或抗体结合,形成抗原抗体复合物,待依序加入酶结合物(酶标记抗体或酶标记抗原)和该酶的底物时,产生呈色反应。呈色程度与检样中待测抗体或抗原的量相关,可据此定性或定量。用于检测精液或宫颈黏液中的抗精子抗体,并行定量分析。方法是以精子或精子抗原提取物放入微试管,加入被测精液或宫颈黏液及抗免疫球蛋白—酶结合物,再加入酶底物观察颜色变化,以自动计算器进行定量测定。
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