1) in situ hybridization histochemistry(ISHH)
组织原位杂交技术
1.
Methods The expression was detected by in situ hybridization histochemistry(ISHH) using labeled digoxigenin probes in the gastric cancer and normal tissues.
方法 利用随机引物法将新片段用地高辛标记为探针 ,通过组织原位杂交技术 (Insituhybridizationhistochemistry ,ISHH)检测其在胃癌及癌旁正常组织中的表达情况。
2) In situ hybridization
组织原位杂交
1.
After screening culture by G418,stable transfected HeLa cell line was established,and the expression of TSARG4 was identified by RT-PCR and in situ hybridization.
RT-PCR和组织原位杂交技术检测TSARG4在稳定转染的HeLa细胞系中的表达。
3) in situ hybridization histochemistry
组织原位杂交
1.
The synapsin Ⅰ mRNA in the hippocampus was observed by in situ hybridization histochemistry.
用组织原位杂交技术观察海马突触素ⅠmRNA表达的变化。
4) In situ hybridization
原位杂交技术
1.
In situ hybridization and Streptavidin-peroxidase(S-P) immunohistochemical method were used to detect the expr.
建立大鼠心肌缺血再灌注损伤模型,测定SOD、GSH-Px活性,应用原位杂交技术和免疫组化S-P法检测HSP70蛋白的表达。
2.
Methods Using in situ hybridization to detect the expressions of Cbfa1 in the synovial tissues of the patients cacroiliac joint with active AS,comparing the results with those in the patients with pelvic fracture and inactive AS;using image analysis to analyze the pictures.
方法通过原位杂交技术检测活动期AS患者与对照组(骨盆骨折患者以及静止期AS患者)骶髂关节滑膜组织中Cbfa1的表达情况,用图像分析系统比较它们的表达差异。
3.
In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of HSP70 mRNA and its protein in 30 c.
建立大鼠心肌缺血再灌注损伤模型,应用原位杂交技术和免疫组化S-P法检测HSP70mRNA及其蛋白的表达。
5) in situ hybridization histochemistry
原位杂交组织化学
1.
Methods: The distribution of PPTAmRNA in the brain of rats and the effect of electroacupuncturing the Zusanli acupoint on its expression in the brain of rats were examined by using in situ hybridization histochemistry.
方法:用原位杂交组织化学的方法观察了正常大鼠脑内PPTAmRNA的正常分布及电针对其表达的影响。
2.
The quantitative changes of TrkB mRNA expression in medial septal nucleus were studied with in situ hybridization histochemistry.
方法:选用Wistar大鼠,分成青年组、老龄组、给药组,采用原位杂交组织化学方法检测大鼠内侧隔核TrkB mRNA含量的变化情况。
3.
Methods In situ hybridization histochemistry staining method with digoxigen-labeled cRNA probe was used.
方法 原位杂交组织化学染色。
6) In situ hybridization
原位杂交组织化学
1.
Methods The expression of NMDAR1 mRNA in the thalamic reticular nuclei was determined by in situ hybridization and image analysis.
方法 应用原位杂交组织化学方法研究戊四氮 (PTZ)致癫痫大鼠和 VNS抗癫痫大鼠丘脑网状核谷氨酸受体 (NMDAR1 ) m RNA的变化。
补充资料：荧光原位杂交
荧光原位杂交
是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条